Nucleic acid chromatography: substitute solid support material for RPC-5 columns.
作者信息
Flanagan J M, Fujimura R K, Jacobson K B
出版信息
Anal Biochem. 1986 Mar;153(2):299-304. doi: 10.1016/0003-2697(86)90096-5.
PMID:3010761
Abstract
Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.
摘要
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