GCU Skin Research Tissue Bank, Department of Life Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, U.K.
Clin Sci (Lond). 2018 Aug 14;132(15):1629-1643. doi: 10.1042/CS20171483. Print 2018 Aug 16.
Impaired wound healing and ulceration caused by diabetes mellitus, is a significant healthcare burden, markedly impairs quality of life for patients, and is the major cause of amputation worldwide. Current experimental approaches used to investigate the complex wound healing process often involve cultures of fibroblasts and/or keratinocytes , which can be limited in terms of complexity and capacity, or utilisation of rodent models in which the mechanisms of wound repair differ substantively from that in humans. However, advances in tissue engineering, and the discovery of strategies to reprogramme adult somatic cells to pluripotency, has led to the possibility of developing models of human skin on a large scale. Generation of induced pluripotent stem cells (iPSCs) from tissues donated by diabetic patients allows the (epi)genetic background of this disease to be studied, and the ability to differentiate iPSCs to multiple cell types found within skin may facilitate the development of more complex skin models; these advances offer key opportunities for improving modelling of wound healing in diabetes, and the development of effective therapeutics for treatment of chronic wounds.
糖尿病引起的伤口愈合和溃疡是一个重大的医疗保健负担，显著降低了患者的生活质量，也是全球截肢的主要原因。目前用于研究复杂伤口愈合过程的实验方法通常涉及成纤维细胞和/或角质细胞的培养，这些方法在复杂性和能力方面可能受到限制，或者使用啮齿动物模型，其中伤口修复的机制与人类有很大的不同。然而，组织工程的进步，以及重新编程成年体体细胞为多能性的策略的发现，使得在大规模上开发人类皮肤模型成为可能。从糖尿病患者捐献的组织中生成诱导多能干细胞（iPSCs），可以研究这种疾病的（表观）遗传背景，并且能够将 iPSCs 分化为皮肤中发现的多种细胞类型，这可能有助于开发更复杂的皮肤模型；这些进展为改善糖尿病伤口愈合模型的建立和治疗慢性伤口的有效治疗方法提供了关键机会。
