含5-卤代尿嘧啶的肽核酸的合成及DNA/RNA互补性研究
Synthesis and DNA/RNA complementation studies of peptide nucleic acids containing 5-halouracils.
作者信息
Liu Chun-Dong, Wang Jian-Hua, Xie Yang, Chen Hang
机构信息
Key Laboratory of Biorheological Science and Technology , Ministry of Education , College of Bioengineering , Chongqing University , Chongqing , 400044 , China . Email:
出版信息
Medchemcomm. 2016 Dec 12;8(2):385-389. doi: 10.1039/c6md00536e. eCollection 2017 Feb 1.
Abstract
The monomers of peptide nucleic acids containing 5-halouracils (5-XU-PNA), incorporated into heptameric PNA in the middle position, have been synthesized. Thermodynamic analyses revealed that the heptameric PNA oligomer with DNA and RNA showed higher duplex stability compared to the unmodified PNA counterpart. NMR studies suggested that the electron withdrawing effect of the halogen atom increased the strength of the XU-A hydrogen bond.
摘要
已合成了含有5-卤代尿嘧啶（5-XU-PNA）的肽核酸单体，并将其掺入七聚体PNA的中间位置。热力学分析表明，与未修饰的PNA对应物相比，具有DNA和RNA的七聚体PNA寡聚物表现出更高的双链稳定性。核磁共振研究表明，卤原子的吸电子效应增加了XU-A氢键的强度。
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