Tomori Takahito, Miyatake Yuya, Sato Yuta, Kanamori Takashi, Masaki Yoshiaki, Ohkubo Akihiro, Sekine Mitsuo, Seio Kohji
Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama 226-8501, Japan.
Org Lett. 2015 Mar 20;17(6):1609-12. doi: 10.1021/acs.orglett.5b00522. Epub 2015 Mar 10.
Synthesis of peptide nucleic acids (PNAs) is reported with new pyridazine-type nucleobases: 3-aminopyridazine (aPz) and 1-aminophthalazine (aPh) as cytosine analogs, and pyridazin-3-one (Pz(O)) and phthalazin-1-one (Ph(O)) as thymine analogs. The PNAs having an aPz or a Pz(O) formed duplexes with each complementary oligodeoxynucleotide forming a base pair with G or A, respectively, as evaluated by using UV melting analyses and circular dichroism (CD) spectra.
据报道，肽核酸（PNA）的合成采用了新型哒嗪类核碱基：作为胞嘧啶类似物的3-氨基哒嗪（aPz）和1-氨基酞嗪（aPh），以及作为胸腺嘧啶类似物的哒嗪-3-酮（Pz(O)）和酞嗪-1-酮（Ph(O)）。通过紫外熔解分析和圆二色性（CD）光谱评估，含有aPz或Pz(O)的PNA与各自互补的寡脱氧核苷酸形成双链体，分别与G或A形成碱基对。
