Costanzo F, Borgatti M, Bartoletti A M, Foà-Tomasi L, Cassai E, Mannini-Palenzona A
Arch Virol. 1986;88(3-4):293-9. doi: 10.1007/BF01310883.
The virus contained in clinical isolates of herpes simplex virus type 1 (HSV-1) which have not undergone previous in vitro passages (new isolates) differs from HSV-1 prototype strains with respect to infected cell glycoprotein pattern, and, most probably efficiency of virus egress at 37 degrees C. The differences can be abolished by lowering the temperature of incubation to 33 degrees C. A few tissue culture passages cause the conversion of the original virus to a virus undistinguishable from HSV-1 prototype strains with respect to the parameters mentioned above.
未经过先前体外传代的单纯疱疹病毒1型（HSV-1）临床分离株（新分离株）所含病毒，在感染细胞糖蛋白模式方面，很可能在37℃时的病毒释放效率方面，与HSV-1原型株有所不同。将孵育温度降至33℃可消除这些差异。几次组织培养传代就会使原始病毒在上述参数方面转变为与HSV-1原型株无法区分的病毒。
