Jackson C L, Shapiro D J
Biochem Biophys Res Commun. 1986 Apr 14;136(1):8-15. doi: 10.1016/0006-291x(86)90869-7.
The regulation of albumin mRNA levels by steroid hormones was examined in a primary Xenopus liver culture system. In the absence of exogenous steroid hormone, albumin mRNA levels declined rapidly in culture. Dexamethasone is required for preservation of albumin mRNA levels in culture and effects a greater than 10 fold induction of albumin mRNA in cultures maintained in steroid hormone-free medium. Estrogen can override the effect of dexamethasone and elicits a decline of greater than 80% in albumin mRNA levels. Our cDNA clones of the mRNAs encoding the two 74,000 dalton and one 72,000 dalton cellular albumins allowed us to show that all three albumin mRNAs were down regulated by estrogen.
在非洲爪蟾原代肝脏培养系统中研究了类固醇激素对白蛋白mRNA水平的调节。在没有外源性类固醇激素的情况下，培养物中白蛋白mRNA水平迅速下降。地塞米松是维持培养物中白蛋白mRNA水平所必需的，并且在无类固醇激素培养基中培养时，它能使白蛋白mRNA诱导增加10倍以上。雌激素可抵消地塞米松的作用，并使白蛋白mRNA水平下降超过80%。我们编码两种74,000道尔顿和一种72,000道尔顿细胞白蛋白的mRNA的cDNA克隆，使我们能够证明所有三种白蛋白mRNA均被雌激素下调。
