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非洲爪蟾白蛋白基因的分离与鉴定：文库扩增导致74K白蛋白基因序列丢失。

Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.

作者信息

May F E, Weber R, Westley B R

出版信息

Nucleic Acids Res. 1982 May 11;10(9):2791-807. doi: 10.1093/nar/10.9.2791.

DOI:10.1093/nar/10.9.2791
PMID:6285309
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320656/
Abstract

The blood of the frog X.laevis contains 2 albumins of 68,000 and 74,000 daltons which are encoded in the liver by two related mRNAs. When an amplified X.laevis DNA library was screened with cloned albumin cDNA only 68,000 dalton albumin gene sequences were isolated. Hybridisation of the albumin cDNA to Southern-blots of Eco R1 digested X.laevis DNA showed that the sequences present in the recombinants did not account for all the fragments which hybridised on the Southern-blots. This indicated that 74K albumin gene sequences exist but that they are not present in the amplified DNA library. A X.laevis genomic library was therefore constructed and screened for albumin genes without amplification. Both 68K and 74K albumin gene sequences were isolated. Recombinants containing 74K albumin gene sequences grew extremely poorly and this probably explains why the 74K albumin sequences were ot isolated from the amplified library. Characterisation of the cloned DNA indicates that there is one sequence coding for the 68K albumin but two different sequences coding for the 75K albumin.

摘要

非洲爪蟾（X.laevis）的血液中含有两种白蛋白，分子量分别为68,000道尔顿和74,000道尔顿，它们由肝脏中的两种相关mRNA编码。当用克隆的白蛋白cDNA筛选扩增的非洲爪蟾DNA文库时，仅分离出了68,000道尔顿白蛋白的基因序列。白蛋白cDNA与经Eco R1消化的非洲爪蟾DNA的Southern印迹杂交显示，重组体中存在的序列并不能解释Southern印迹上所有杂交的片段。这表明74K白蛋白基因序列存在，但在扩增的DNA文库中不存在。因此构建了一个非洲爪蟾基因组文库，并在不进行扩增的情况下筛选白蛋白基因。68K和74K白蛋白基因序列均被分离出来。含有74K白蛋白基因序列的重组体生长极其缓慢，这可能解释了为什么74K白蛋白序列未从扩增文库中分离出来。对克隆DNA的表征表明，有一个序列编码68K白蛋白，但有两个不同的序列编码75K白蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/cc6c661fa0e5/nar00378-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/11d244a6a043/nar00378-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/d612f073928f/nar00378-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/6b70378b3fb7/nar00378-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/cc6c661fa0e5/nar00378-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/11d244a6a043/nar00378-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/d612f073928f/nar00378-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/6b70378b3fb7/nar00378-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c308/320656/cc6c661fa0e5/nar00378-0043-a.jpg

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Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.非洲爪蟾白蛋白基因的分离与鉴定：文库扩增导致74K白蛋白基因序列丢失。
Nucleic Acids Res. 1982 May 11;10(9):2791-807. doi: 10.1093/nar/10.9.2791.
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引用本文的文献

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Mouse mammary tumour virus related sequences are present in human DNA.小鼠乳腺肿瘤病毒相关序列存在于人类DNA中。

本文引用的文献

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Chromosomal arrangement of the chicken beta-type globin genes.鸡β型珠蛋白基因的染色体排列
Cell. 1981 Jun;24(3):669-77. doi: 10.1016/0092-8674(81)90093-3.
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Vitellogenesis and the vitellogenin gene family.卵黄生成与卵黄蛋白原基因家族
Science. 1981 Apr 17;212(4492):298-304. doi: 10.1126/science.7209528.
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The structure and evolution of the human beta-globin gene family.人类β-珠蛋白基因家族的结构与进化
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4
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.非洲爪蟾的卵黄蛋白原B2基因：分离、体外转录及其与其他卵黄蛋白原基因的关系
Nucleic Acids Res. 1983 May 25;11(10):2979-97. doi: 10.1093/nar/11.10.2979.
5
Vitellogenin genes A1 and B1 are linked in the Xenopus laevis genome.卵黄蛋白原基因A1和B1在非洲爪蟾基因组中是连锁的。
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6832-6. doi: 10.1073/pnas.79.22.6832.
6
The primary structure of the larval beta 1-globin gene of Xenopus laevis and its flanking regions.非洲爪蟾幼虫β1-珠蛋白基因的一级结构及其侧翼区域。
Nucleic Acids Res. 1984 Oct 25;12(20):7705-19. doi: 10.1093/nar/12.20.7705.
7
DNA sequence of the first exon and 5' flanking region of the 68-K serum albumin gene of Xenopus laevis.非洲爪蟾68-K血清白蛋白基因第一个外显子及5'侧翼区域的DNA序列
Nucleic Acids Res. 1987 Jul 24;15(14):5889. doi: 10.1093/nar/15.14.5889.
8
A novel nuclear transcription system which responds correctly to cloned estrogen receptor.一种对克隆的雌激素受体能做出正确反应的新型核转录系统。
Nucleic Acids Res. 1988 Nov 25;16(22):10469-76. doi: 10.1093/nar/16.22.10469.
9
Globin evolution in the genus Xenopus: comparative analysis of cDNAs coding for adult globin polypeptides of Xenopus borealis and Xenopus tropicalis.非洲爪蟾属中的珠蛋白进化：北方爪蟾和热带爪蟾成年珠蛋白多肽编码cDNA的比较分析。
J Mol Evol. 1986;23(3):211-23. doi: 10.1007/BF02115578.
10
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Mol Cell Biol. 1986 Feb;6(2):393-403. doi: 10.1128/mcb.6.2.393-403.1986.
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4
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5
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Nucleic Acids Res. 1981 Oct 10;9(19):4981-95. doi: 10.1093/nar/9.19.4981.
6
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J Biol Chem. 1981 Feb 25;256(4):1960-7.
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Cell. 1980 Apr;19(4):959-72. doi: 10.1016/0092-8674(80)90087-2.
10
Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.非洲爪蟾核糖体蛋白基因：重组cDNA克隆的分离及基因组组织研究
Nucleic Acids Res. 1981 Mar 11;9(5):1069-86. doi: 10.1093/nar/9.5.1069.