1 Technological Plasma Research Unit, Department of Physics, Faculty of Science.
2 Department of Biology, Faculty of Science, and.
J Food Prot. 2018 Sep;81(9):1503-1507. doi: 10.4315/0362-028X.JFP-18-062.
A low-pressure capacitively coupled discharge was used to study antimicrobial treatment in herbal tea. Ambient air with a relative humidity of 40% was used as a precursor gas and fed into the chamber via a perforated power electrode. An electrical discharge plasma was produced at a radio frequency of 10 kHz and power of 80 W. The operating pressure during treatment was kept constant at 260 Pa. The target microorganisms, Escherichia coli and Staphylococcus aureus, isolated from the herbal tea were inoculated on nutrient agar petri dishes and exposed to the plasma for 0.5, 1.0, 1.5, and 2.0 min. All treatments were carried out in triplicate for different exposure times to calculate the D-value by the enumeration method. D-values of 0.73 and 0.67 min were obtained corresponding to E. coli and S. aureus reduction, respectively.
采用低压容性耦合放电研究了草药的抗菌处理。相对湿度为 40%的环境空气作为前体气体,通过穿孔功率电极送入腔室。在 10 kHz 的射频和 80 W 的功率下产生电放电等离子体。处理过程中的工作压力保持在 260 Pa 不变。从草药中分离出的目标微生物大肠杆菌和金黄色葡萄球菌接种在营养琼脂培养皿上,并暴露于等离子体中 0.5、1.0、1.5 和 2.0 分钟。为了计算通过计数法获得的 D 值,针对不同的暴露时间重复进行了三次处理。大肠杆菌和金黄色葡萄球菌的 D 值分别为 0.73 和 0.67 分钟。