液体中浮游金黄色葡萄球菌和大肠杆菌的直流空气-液体放电等离子体的体外抗菌作用及机制。
In vitro antimicrobial effects and mechanisms of direct current air-liquid discharge plasma on planktonic Staphylococcus aureus and Escherichia coli in liquids.
机构信息
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, Anhui Province 230009, PR China; Institute of Plasma Physics, Chinese Academy of Sciences, P. O. Box 1126, Hefei 230031, PR China; Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China; Anhui Province Key Laboratory of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China.
Institute of Plasma Physics, Chinese Academy of Sciences, P. O. Box 1126, Hefei 230031, PR China; Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China; Anhui Province Key Laboratory of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China.
出版信息
Bioelectrochemistry. 2018 Jun;121:125-134. doi: 10.1016/j.bioelechem.2018.01.012. Epub 2018 Feb 3.
The direct inactivation effects of an atmospheric pressure direct current (DC) air plasma against planktonic Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in aqueous solution are investigated in vitro. Upon plasma treatment, extensively analyses on cell culturability, metabolic capacity, membrane integrity, surface morphology, cellular proteins, nucleic acids and intracellular reactive oxygen species (ROS) for both bacterial species were carried out and significant antimicrobial effects observed. Compared with the cellular culturability, a sub-lethal viable but non-culturable (VBNC) state was induced while more S. aureus entered this state than E. coli. Damaged bacterial outer structures were observed and the total concentrations of cellular protein and nucleic acid decreased for both bacteria after plasma treatment. The plasma-induced aqueous reactive species (RS) and intracellular ROS might produce detrimental effects to the bacteria, while S. aureus was less susceptible to the discharge after a 20-min exposure compared to E. coli.
体外研究了常压直流电（DC）空气等离子体对浮游金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）在水溶液中的直接灭活作用。在等离子体处理后，对两种细菌的细胞培养能力、代谢能力、膜完整性、表面形态、细胞蛋白质、核酸和细胞内活性氧（ROS）进行了广泛分析，观察到了显著的抗菌作用。与细胞培养能力相比，诱导了一种亚致死性的活但非可培养（VBNC）状态，而 S. aureus 进入这种状态的比例高于 E. coli。处理后观察到细菌外层结构受损，两种细菌的细胞总蛋白和核酸浓度均下降。等离子体诱导的水相活性物质（RS）和细胞内 ROS 可能对细菌产生有害影响，而与 E. coli 相比，金黄色葡萄球菌在 20 分钟暴露后对放电的敏感性较低。
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