Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering , Shangqiu Normal University , Shangqiu 476000 , P. R. China.
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University , Liaoning 113001 , P. R. China.
Anal Chem. 2018 Nov 6;90(21):12449-12455. doi: 10.1021/acs.analchem.8b01546. Epub 2018 Oct 23.
Metal-organic coordination polymers (MOCPs) have been emerging as very attractive nanomaterials due to their tunable nature and diverse applications. Herein, using Tb as the luminescence center, 1,3,5-benzenetricarboxylate (BTC) as building block and Cu as the signal modulator as well as a recognition unit, we propose a novel and effective lanthanide functionalized MOCP (LMOCP) fluorescent sensor (Cu-BTC/Tb) for amyloid β-peptide (Aβ) monomer, a biomarker for Alzheimer disease (AD). Specifically, Cu-BTC/Tb, created by postsynthesis modification strategy under room temperature, is almost nonemissive due to the quenching effect of Cu in the MOCP, exhilaratingly, the presence of Aβ triggered a significant emission enhancement of Cu-BTC/Tb assay due to the high binding affinity of Aβ for Cu and the subsequent suppression of the quenching effect. In the assay, this LMOCP sensor shows high sensitivity with detection limit of 0.3 nM. Due to its capability to eliminate autofluorescence, Cu-BTC/Tb was also applied to the time-gated detection of Aβ in human plasma with promising results. This work presents a novel strategy for the construction of functional luminescent LMOCP for sensitively turn-on fluorescent sensing of Aβ. We believe the proposed strategy would inspire the development of various LMOCP-based fluorescent assays or medical imaging platforms for advanced biological implementations.
金属有机配位聚合物（MOCPs）由于其可调谐性和多样化的应用而成为非常有吸引力的纳米材料。在此，我们以 Tb 为发光中心，以 1,3,5-苯三甲酸（BTC）为构筑块，以 Cu 为信号调节剂和识别单元，提出了一种新型有效的镧系功能化 MOCP（LMOCP）荧光传感器（Cu-BTC/Tb），用于检测淀粉样β肽（Aβ）单体，这是阿尔茨海默病（AD）的生物标志物。具体来说，Cu-BTC/Tb 是通过室温下的后合成修饰策略制备的，由于 MOCP 中 Cu 的猝灭效应，其几乎没有发光性，令人振奋的是，由于 Aβ与 Cu 具有高结合亲和力，以及随后抑制猝灭效应，Aβ的存在引发了 Cu-BTC/Tb 测定的显著发射增强。在测定中，这种 LMOCP 传感器具有 0.3 nM 的检测限，表现出高灵敏度。由于其能够消除自发荧光，Cu-BTC/Tb 还被应用于人血浆中 Aβ的时间门控检测，结果令人满意。这项工作提出了一种构建功能发光 LMOCP 的新策略，用于灵敏地开启 Aβ的荧光传感。我们相信，所提出的策略将激发各种基于 LMOCP 的荧光分析或医学成像平台的发展，以实现先进的生物学应用。
