Dehdari Vais Rezvan, Yadegari Hossein, Heli Hossein, Sattarahmady Naghmeh
PhD Candidate, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
PhD, Department of Mechanical and Materials Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.
J Biomed Phys Eng. 2021 Apr 1;11(2):215-228. doi: 10.31661/jbpe.v0i0.1070. eCollection 2021 Apr.
Alzheimer's disease (AD) is a common form of dementia, characterized by production and deposition of β-amyloid peptide in the brain. Thus, β-amyloid peptide is a potentially promising biomarker used to diagnose and monitor the progression of AD.
The study aims to develop a biosensor based on a molecularly imprinted poly-pyrrole for detection of β-amyloid.
In this experimental study, an imprinted poly-pyrrole was employed as an artificial receptor synthesized by electro-polymerization of pyrrole on screen-printed carbon electrodes in the presence of β-amyloid. β-amyloid acts as a molecular template within the polymer. The biosensor was evaluated by cyclic voltammetry using ferro/ferricyanide marker. The parameters influencing the biosensor performance, including electro-polymerization cycle umbers and β-amyloid binding time were optimized to achieve the best biosensor sensitivity.
The β-amyloid binding affinity with the biosensor surface was evaluated by the Freundlich isotherm, and Freundlich constant and exponent were obtained as 0.22 ng mL and 10.60, respectively. The biosensor demonstrated a detection limit of 1.2 pg mL. The biosensor was applied for β-amyloid determination in artificial cerebrospinal fluid.
The biosensor is applicable for early Alzheimer's disease detection.
阿尔茨海默病（AD）是一种常见的痴呆形式，其特征是大脑中β-淀粉样肽的产生和沉积。因此，β-淀粉样肽是一种用于诊断和监测AD进展的潜在有前景的生物标志物。
本研究旨在开发一种基于分子印迹聚吡咯的生物传感器用于检测β-淀粉样蛋白。
在本实验研究中，印迹聚吡咯被用作人工受体，通过在β-淀粉样蛋白存在的情况下在丝网印刷碳电极上对吡咯进行电聚合来合成。β-淀粉样蛋白在聚合物中充当分子模板。使用铁氰化亚铁/铁氰化铁标记物通过循环伏安法对生物传感器进行评估。对影响生物传感器性能的参数，包括电聚合循环次数和β-淀粉样蛋白结合时间进行优化，以实现最佳的生物传感器灵敏度。
通过弗罗因德利希等温线评估β-淀粉样蛋白与生物传感器表面的结合亲和力，得到弗罗因德利希常数和指数分别为0.22 ng/mL和10.60。该生物传感器的检测限为1.2 pg/mL。该生物传感器用于人工脑脊液中β-淀粉样蛋白的测定。
该生物传感器适用于早期阿尔茨海默病的检测。
