Guo Yuxin, Chen Chong, Xie Tong, Cui Wei, Meng Haotian, Jin Xiaoye, Zhu Bofeng
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, People's Republic of China.
Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, People's Republic of China.
R Soc Open Sci. 2018 Jun 13;5(6):172089. doi: 10.1098/rsos.172089. eCollection 2018 Jun.
Short tandem repeats (STRs) with a high level of polymorphisms and convenient detection method play an indispensable role in human population and forensic genetics. Recently, we detected the 21 autosomal non-combined DNA index system (non-CODIS) STR loci in a Kyrgyz ethnic group, calculated their forensic parameters and analysed its genetic relationships with reference populations from China. In total, 168 alleles were observed at 21 non-CODIS STRs with corresponding allelic frequencies from 0.0016 to 0.4788. No significant deviations at these STRs were observed from the Hardy-Weinberg equilibrium. The values of cumulative power of discrimination and probability of exclusion for all the 21 non-CODIS STRs were 0.99999999999999999998835 and 0.9999994002, respectively. Furthermore, the analyses of phylogenetic trees, genetic distances and interpopulation differentiations demonstrated that the Kyrgyz group had relatively close genetic relationships with the Uygur and Kazak groups. These 21 non-CODIS STRs were characterized by high genetic diversities in the Kyrgyz group and could be applied as a robust tool for individual identification and kinship testing in forensic sciences.
具有高度多态性且检测方法便捷的短串联重复序列（STR）在人类群体遗传学和法医遗传学中发挥着不可或缺的作用。最近，我们检测了吉尔吉斯族群体中的21个常染色体非联合DNA索引系统（非CODIS）STR基因座，计算了它们的法医参数，并分析了其与中国参考群体的遗传关系。在21个非CODIS STR基因座上共观察到168个等位基因，相应的等位基因频率为0.0016至0.4788。在这些STR基因座上未观察到显著偏离哈迪-温伯格平衡的情况。所有21个非CODIS STR基因座的累积鉴别力值和排除概率分别为0.99999999999999999998835和0.9999994002。此外，系统发育树、遗传距离和群体间分化分析表明，吉尔吉斯群体与维吾尔族和哈萨克族群体具有相对密切的遗传关系。这21个非CODIS STR基因座在吉尔吉斯群体中具有高度的遗传多样性，可作为法医科学中个体识别和亲属关系检测的有力工具。
