Zhu Bo-Feng, Zhang Yu-Dang, Shen Chun-Mei, Du Wei-An, Liu Wen-Juan, Meng Hao-Tian, Wang Hong-Dan, Yang Guang, Jin Rui, Yang Chun-Hua, Yan Jiang-Wei, Bie Xiao-Hua
Research Center of Stomatology, Stomatology Hospital, Xi'an Jiaotong University, Xi'an, China.
Electrophoresis. 2015 Jan;36(2):271-6. doi: 10.1002/elps.201400333. Epub 2014 Dec 17.
In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.
在本研究中，我们描述了对新设计的STR复合扩增系统AGCU 21+1 STR试剂盒进行的发育验证分析。该试剂盒包含一个性别决定基因座牙釉蛋白和21个非联合DNA索引系统STR基因座，即D6S474、D12ATA63、D22S1045、D10S1248、D1S1677、D11S4463、D1S1627、D3S4529、D2S441、D6S1017、D4S2408、D19S433、D17S1301、D1GATA113、D18S853、D20S482、D14S1434、D9S1122、D2S1776、D10S1435和D5S2500。根据DNA分析方法科学工作组（SWGDAM）发布的修订验证指南，通过一系列测试对21+1试剂盒进行了验证，包括优化的PCR条件、灵敏度、精密度和准确性、拖带率、DNA混合物、抑制剂和物种特异性。我们在本研究中的结果表明，该试剂盒是法医应用的有用工具。
