不同产地黄芩药材药用成分含量的地理变异研究

[Study on geographical variation of medicinal ingredients contents in Scutellaria baicalensis from different provenances].

作者信息

Li Yong-Jie, Liu Rong-Xiu, Tian Wen-Cang, Wang Xue-Sen, Hou Zhi-Yan, Meng Lei, Song Guo-Hu, Zhang Xue-Wen, Wei Sheng-Li

机构信息

College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.

Chengde Pharmaceutical Co., Ltd.， Affiliated to China National Pharmaceutical Corporation， Chengde 067000， China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(13):2664-2669. doi: 10.19540/j.cnki.cjcmm.20180510.009.

DOI:10.19540/j.cnki.cjcmm.20180510.009

PMID:30111014

Abstract

In order to investigate the genetic difference on medicinal components of Scutellaria baicalensis from different provenances on the genetic difference, the S. baicalensis provenance tests were arranged by randomized block design.Excavating the crude drugs that have been growing for three years, with the same drying process, the content of baicalin, baicalein, wogonoside, wogonin and laminarin A in S. baicalensis were detected by HPLC, and then the data were analyzed. The results indicated that the content of baicalin in different provenances of S. baicalensis was significantly different (<0.05), while the variation of baicalein reached extremely significant level (<0.01). Cluster analysis showed that if the distance was divided by 5.0, the provenances in Chengde, Hebei province were divided into two independent populations, while the other two populations had large geographic spans. The results show that the significant geographical variations exist in the content of medicinal components in S. baicalensis. The study laid a theoretical foundation of provenance selection of S. baicalensis.

摘要

为了研究不同产地黄芩药用成分的遗传差异，采用随机区组设计进行黄芩产地试验。挖掘生长三年的药材，经相同干燥处理后，用高效液相色谱法检测黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素和千层纸素A的含量，然后进行数据分析。结果表明，不同产地黄芩中黄芩苷含量差异显著（<0.05），黄芩素含量变异达到极显著水平（<0.01）。聚类分析表明，若以距离5.0划分，河北省承德市的产地被分为两个独立群体，另外两个群体地理跨度较大。结果表明，黄芩药用成分含量存在显著的地理变异。该研究为黄芩的产地选择奠定了理论基础。