Choi Seon, Lee Ji Hee, Kang Joo Won, Choe Han Na, Seong Chi Nam
1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
2Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Jeongeup 56212, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3266-3272. doi: 10.1099/ijsem.0.002975. Epub 2018 Aug 16.
A Gram-stain-negative, non-spore-forming, rod-shaped, aerobic and diffusible yellow-coloured bacterial strain, designated strain ECD12 was isolated from a seaweed, Ecklonia cava. The isolate required sea salts for growth. Catalase-positive and oxidase-negative. A phylogenetic tree based on 16S rRNA gene sequences showed that strain ECD12 formed an evolutionary lineage within the radiation enclosing the members of genera Spongiibacterium and Flagellimonas sharing the highest similarity to Flagellimonas eckloniae DOKDO007 (96.8 % 16S rRNA gene sequence similarity) followed by Spongiibacterium pacificum SW169 (96.4 %) and Spongiibacterium flavum DSM 22638 (96.1 %). The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. The new isolate contained MK-6 as the only isoprenoid quinone and phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids as the major polar lipids. The genomic DNA G+C content is 39 mol%. A number of phenotypic characteristics such as the production of diffusible pigment distinguished strain ECD12 from the related species. On the basis of the evidence presented in this study, a novel species, Flagellimonas aquimarina sp. nov., is proposed for strain ECD12 (=KCTC 52351=JCM 32292). Based on the sequence similarity, phylogenetic relationship and common morphological, physiological and chemical characters among the members of the genera Spongiibacterium and Flagellimonas, it is recommended that the two genera are combined into a single genus. Thus, transfer of S. flavumYoon and Oh 2012 and S. pacificum Gao et al. 2015 to the genus FlagellimonasBae et al. 2007 as Flagellimonas flava comb. nov. and Flagellimonas pacifica comb. nov., respectively, is also proposed.
从海藻昆布（Ecklonia cava）中分离出一株革兰氏染色阴性、不产芽孢、杆状、需氧且能产生扩散性黄色色素的菌株，命名为ECD12。该分离株生长需要海盐。过氧化氢酶阳性，氧化酶阴性。基于16S rRNA基因序列构建的系统发育树表明，菌株ECD12在包含海绵杆菌属（Spongiibacterium）和鞭毛单胞菌属（Flagellimonas）成员的辐射分支内形成了一个进化谱系，与海带鞭毛单胞菌（Flagellimonas eckloniae）DOKDO007的相似性最高（16S rRNA基因序列相似性为96.8%），其次是太平洋海绵杆菌（Spongiibacterium pacificum）SW169（96.4%）和黄色海绵杆菌（Spongiibacterium flavum）DSM 22638（96.1%）。主要脂肪酸为异-C15 : 0、异-C17 : 0 3-OH和异-C15 : 1 G。该新分离株含有MK-6作为唯一的类异戊二烯醌，以及磷脂酰乙醇胺、两种未鉴定的氨基脂和两种未鉴定的脂类作为主要极性脂类。基因组DNA的G+C含量为39 mol%。一些表型特征，如产生扩散性色素，使菌株ECD12与相关物种区分开来。基于本研究提供的证据，为菌株ECD12（=KCTC 52351=JCM 32292）提出了一个新物种，即海洋鞭毛单胞菌（Flagellimonas aquimarina sp. nov.）。基于海绵杆菌属和鞭毛单胞菌属成员之间的序列相似性、系统发育关系以及共同的形态、生理和化学特征，建议将这两个属合并为一个属。因此，还提议将2012年尹和吴的黄色海绵杆菌（S. flavum）以及2015年高等人的太平洋海绵杆菌（S. pacificum）分别转移到2007年裴等人的鞭毛单胞菌属，分别命名为黄色鞭毛单胞菌（Flagellimonas flava comb. nov.）和太平洋鞭毛单胞菌（Flagellimonas pacifica comb. nov.）。
