Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, PR China.
School of Public Health and Management, Chongqing Medical University, Chongqing 400016, PR China.
Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.004982.
An aerobic, Gram-stain-negative, rod-shaped and non-motile strain (XY-359) was isolated from the mouth of a marine invertebrate species from the South China Sea. It grew at pH 6.0-8.5 (optimum, pH 7.5), at 15-37 °C (optimum, 30 °C) and in the presence of 0.5-4.5 % (w/v) NaCl (optimum, 2.5 %). It could not hydrolyse Tweens 20, 40, 60 or 80 and no flexirubin-type pigments were produced. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, six unidentified phospholipids and two unidentified polar lipids. The major fatty acids were iso-C 3-OH, iso-C G and iso-C 3-OH. The respiratory quinone was MK-6. Strain XY-359 showed the greatest degree of 16S rRNA sequence similarity to AsT0115 (96.54 %), followed by DSM 22638 (96.27 %). Phylogenetic analysis based on 16S rRNA gene sequences and 31 core genes indicated that strain XY-359 belongs to the genus . The genome size of strain XY-359 was 4 207 872 bp, with 39.1 mol% of DNA G+C content. The average nucleotide identity and digital DNA-DNA hybridization values between strain XY-359 and AsT0115 were 74.58 % and 18.5 %, respectively, and those between strain XY-359 and DSM 22638 were 74.2 % and 18.3 %. The combined phenotypic, chemotaxonomic and phylogenetic data suggest that strain XY-359 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XY-359 (=MCCC 1K03658 =KCTC 72218). Moreover, based on the proposal of nesting and within by García (Validation List No. 193) and the analyses of phylogenetic trees and average amino acid identities in this study, the transfers of , and to the genus as comb. nov., nom. nov. and nom. nov., respectively, are proposed, with an emended description of the genus .
从南海一种海洋无脊椎动物的口中分离到一株需氧、革兰氏阴性、杆状和非运动的菌株（XY-359）。它在 pH 值 6.0-8.5（最佳，pH 值 7.5）、15-37°C（最佳，30°C）和 0.5-4.5%（w/v）NaCl（最佳，2.5%）的条件下生长。它不能水解吐温 20、40、60 或 80，也不产生柔韧型色素。主要极性脂是磷脂酰乙醇胺、一种未鉴定的氨基脂、六种未鉴定的磷脂和两种未鉴定的极性脂。主要脂肪酸是 iso-C 3-OH、iso-C G 和 iso-C 3-OH。呼吸醌是 MK-6。菌株 XY-359 与 AsT0115（96.54%）的 16S rRNA 序列相似性最高，其次是 DSM 22638（96.27%）。基于 16S rRNA 基因序列和 31 个核心基因的系统发育分析表明，菌株 XY-359 属于 属。菌株 XY-359 的基因组大小为 4 207 872 bp，DNA G+C 含量为 39.1 mol%。菌株 XY-359 与 AsT0115 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 74.58%和 18.5%，与 DSM 22638 的分别为 74.2%和 18.3%。综合表型、化学分类和系统发育数据表明，菌株 XY-359 代表 属的一个新种，建议命名为. sp. nov.。模式菌株为 XY-359（=MCCC 1K03658=KCTC 72218）。此外，根据 García 提出的嵌套 和 在 中的提议（第 193 号验证清单）以及本研究中系统发育树和平均氨基酸同一性的分析，提议将 、 和 转移到 属中，分别作为 comb. nov.、 nom. nov. 和 nom. nov.，并对 属进行了修订描述。
