Cao Wenrui, Deng Xingyu, Jiang Mingyu, Zeng Zhigang, Chang Fengming
Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
College of Earth Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China.
Microorganisms. 2023 Jun 14;11(6):1580. doi: 10.3390/microorganisms11061580.
Two strains, 81s02 and 334s03, were isolated from the sediment core near the hydrothermal field of southern Okinawa Trough. The cells of both strains were observed to be rod-shaped, non-gliding, Gram-staining negative, yellow-pigmented, facultatively anaerobic, catalase and oxidase positive, and showing optimum growth at 30 °C and pH 7.5. The strains 81s02 and 334s03 were able to tolerate up to 10% and 9% (/) NaCl concentration, respectively. Based on phylogenomic analysis, the average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) values between the two strains and the nearest phylogenetic neighbors of the genus were in range of 78.0-86.3% and 21.5-33.9%, respectively. The strains 81s02 and 334s03 shared 98.1% 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.4-81.5% ANIb, 85.5-85.6% ANIm and 25.4% dDDH values calculated using whole genome sequences. The strains 81s02 and 334s03 shared the highest 16S rRNA gene sequence similarity to SMK-108 (98.7%) and BC31-1-A7 (98.8%), respectively. The major fatty acid of strains 81s02 and 334s03 were identified similarly as iso-C, iso-C 3-OH and iso-C G, and the major polar lipids of the both strains consisted of phosphatidylethanolamine and two unidentified lipids. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains 81s02 and 334s03 were determined to be 41.6 and 41.9 mol%, respectively. Based on the phylogenetic and phenotypic characteristics, both strains are considered to represent two novel species of the genus , and the names sp. nov. and sp. nov. are proposed for strains 81s02 (=KCTC 92889 = MCCC 1K08502) and 334s03 (=KCTC 92890 = MCCC 1K08503).
从冲绳海槽南部热液区附近的沉积物岩芯中分离出两株菌株，81s02和334s03。观察到这两株菌株的细胞均为杆状，不滑动，革兰氏染色阴性，黄色色素沉着，兼性厌氧，过氧化氢酶和氧化酶阳性，在30℃和pH 7.5条件下生长最佳。菌株81s02和334s03分别能够耐受高达10%和9%（/）的NaCl浓度。基于系统基因组分析，这两株菌株与该属最近的系统发育邻居之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别在78.0-86.3%和21.5-33.9%范围内。菌株81s02和334s03的16S rRNA基因序列相似度为98.1%，但根据使用全基因组序列计算的81.4-81.5% ANIb、85.5-85.6% ANIm和25.4% dDDH值，它们被鉴定为两个不同的物种。菌株81s02和334s03的16S rRNA基因序列分别与SMK-108（98.7%）和BC31-1-A7（98.
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