Institute for Microbial Biotechnology and Metagenomics (IMBM), University of the Western Cape, Robert Sobukwe Road, Bellville, Cape Town, South Africa.
L'Oréal Research and Innovation, 1 Avenue Eugène Schueller, 93600, Aulnay sous Bois, France.
Sci Rep. 2018 Aug 16;8(1):12265. doi: 10.1038/s41598-018-30705-1.
Recent skin metagenomic studies have investigated the harbored viral diversity and its possible influence on healthy skin microbial populations, and tried to establish global patterns of skin-phage evolution. However, the detail associated with the phages that potentially play a role in skin health has not been investigated. While skin metagenome and -metavirome studies have indicated that the skin virome is highly site specific and shows marked interpersonal variation, they have not assessed the presence/absence of individual phages. Here, we took a semi-culture independent approach (metaviromic) to better understand the composition of phage communities on skin from South African study participants. Our data set adds over 130 new phage species of the skin to existing databases. We demonstrated that identical phages were present on different individuals and in different body sites, and we conducted a detailed analysis of the structural organization of these phages. We further found that a bacteriophage related to the Staphylococcus capitis phage Stb20 may be a common skin commensal virus potentially regulating its host and its activities on the skin.
最近的皮肤宏基因组研究调查了栖息病毒的多样性及其对健康皮肤微生物群的可能影响，并试图建立皮肤噬菌体进化的全球模式。然而，与可能在皮肤健康中发挥作用的噬菌体相关的细节尚未被研究。虽然皮肤宏基因组和宏病毒组研究表明，皮肤病毒组具有高度的特定部位特征，并表现出明显的人际间变异，但它们并未评估单个噬菌体的存在/缺失。在这里，我们采用了一种半培养独立的方法（宏病毒组学）来更好地了解南非研究参与者皮肤噬菌体群落的组成。我们的数据集为现有的数据库增加了超过 130 种新的皮肤噬菌体。我们证明了相同的噬菌体存在于不同个体和不同身体部位，并且对这些噬菌体的结构组织进行了详细分析。我们进一步发现，一种与头葡萄球菌噬菌体 Stb20 相关的噬菌体可能是一种常见的皮肤共生病毒，它可能调节其宿主及其在皮肤上的活动。
