Valente Luca G, Pitton Melissa, Fürholz Monika, Oberhaensli Simone, Bruggmann Rémy, Leib Stephen L, Jakob Stephan M, Resch Grégory, Que Yok-Ai, Cameron David R
Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
FEMS Microbes. 2021 Mar 30;2:xtab003. doi: 10.1093/femsmc/xtab003. eCollection 2021.
Phage therapy might be a useful approach for the treatment of nosocomial infections; however, only few lytic phages suitable for this application are available for the opportunistic pathogen, . In the current study, we developed an efficient method to isolate bacteriophages present within the human skin microbiome, by using niche-specific as the host for phage propagation. was identified on the forehead of 92% of human subjects tested. These isolates were then used to propagate phages present in the same skin sample. Plaques were observable on bacterial lawns in 46% of the cases where was isolated. A total of eight phage genomes were genetically characterized, including the previously described phage 456. A total of six phage sequences were unique, and spanned each of the major staphylococcal phage families; Siphoviridae ( = 3), Podoviridae ( = 1) and Myoviridae ( = 2). One of the myoviruses (vB_SepM_BE06) was identified on the skin of three different humans. Comparative analysis identified novel genes including a putative N-acetylmuramoyl-L-alanine amidase gene. The host-range of each unique phage was characterized using a panel of diverse staphylococcal strains ( = 78). None of the newly isolated phages infected more than 52% of the strains tested ( = 44), and non- strains where rarely infected, highlighting the narrow host-range of the phages. One of the phages (vB_SepM_BE04) was capable of killing staphylococcal cells within biofilms formed on polyurethane catheters. Uncovering a richer diversity of available phages will likely improve our understanding of -phage interactions, which will be important for future therapy.
噬菌体疗法可能是治疗医院感染的一种有用方法；然而，对于这种机会性病原体，仅有少数适合该应用的裂解性噬菌体可用。在本研究中，我们开发了一种有效的方法，通过使用特定生态位的[细菌名称]作为噬菌体繁殖的宿主，来分离人类皮肤微生物群中存在的噬菌体。在92%接受测试的人类受试者的前额上鉴定出了[细菌名称]。然后使用这些分离株来繁殖同一皮肤样本中存在的噬菌体。在分离出[细菌名称]的病例中，46%的情况下在细菌菌苔上可观察到噬菌斑。总共对八个噬菌体基因组进行了遗传特征分析，包括先前描述的噬菌体456。共有六个噬菌体序列是独特的，涵盖了葡萄球菌主要噬菌体家族的每一个；长尾噬菌体科（n = 3）、短尾噬菌体科（n = 1）和肌尾噬菌体科（n = 2）。其中一种肌尾病毒（vB_SepM_BE06）在三个不同人的皮肤上被鉴定出来。比较分析鉴定出了新基因，包括一个假定的N - 乙酰胞壁酰 - L - 丙氨酸酰胺酶基因。使用一组不同的葡萄球菌菌株（n = 78）对每个独特噬菌体的宿主范围进行了表征。新分离的噬菌体中没有一个能感染超过所测试菌株（n = 44）的52%，并且非[细菌名称]菌株很少被感染，这突出了噬菌体宿主范围狭窄的特点。其中一种噬菌体（vB_SepM_BE04）能够杀死在聚氨酯导管上形成的生物膜内的葡萄球菌细胞。发现更丰富多样的可用噬菌体可能会增进我们对[细菌名称] - 噬菌体相互作用的理解，这对未来的治疗将很重要。
