Cigan A M, Donahue T F
Gene. 1986;41(2-3):343-8. doi: 10.1016/0378-1119(86)90118-6.
We have isolated three distinct tRNAimet genes from a yeast DNA clone bank. The complete sequence of two shows that these genes are colinear with the mature tRNAimet and supports the RNA sequence of tRNAimet. Southern analysis of yeast genomic DNA indicates the presence of four copies of tRNAimet gene per haploid genome.
我们从酵母DNA克隆文库中分离出了三个不同的甲硫氨酸tRNA基因。其中两个基因的完整序列表明,这些基因与成熟的甲硫氨酸tRNA呈共线性,并支持甲硫氨酸tRNA的RNA序列。对酵母基因组DNA的Southern分析表明,单倍体基因组中存在四个甲硫氨酸tRNA基因拷贝。