Pochart P, Agoutin B, Fix C, Keith G, Heyman T
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
Nucleic Acids Res. 1993 Apr 11;21(7):1517-21. doi: 10.1093/nar/21.7.1517.
The analysis of the tRNAs associated to the virus-like particles produced by the Ty1 element revealed the specific packaging of three major tRNA species, in about equal amounts: the replication primer initiator tRNA(Met), the tRNA(Ser)AGA and a tRNA undetected until now as an expressed species in yeast. The latter tRNA is coded by the already described tDNA(Ser)GCT. This tRNA is enriched more than 150 fold in the particles as compared to its content in total cellular tRNA where it represents less than 0.1% (initiator tRNA(Met) and tRNA(Ser)AGA being 11 and 4 fold enriched respectively). This tRNA is the only species coded by the tDNA(Ser)GCT gene which is found in three copies per genome since no other corresponding expressed tRNA could be detected. This gene is thus very poorly expressed. The high concentration of tRNA(Ser)GCU in the particles compared to its very low cellular content led us to consider its possible implication in Ty specific processes.
对由Ty1元件产生的病毒样颗粒相关的tRNA进行分析，结果显示三种主要tRNA种类以大致相等的量进行特异性包装：复制引物起始tRNA（Met）、tRNA（Ser）AGA以及一种至今在酵母中未作为表达种类被检测到的tRNA。后一种tRNA由已描述的tDNA（Ser）GCT编码。与它在总细胞tRNA中的含量相比（其在总细胞tRNA中占比不到0.1%，起始tRNA（Met）和tRNA（Ser）AGA分别富集11倍和4倍），这种tRNA在颗粒中的富集程度超过150倍。这种tRNA是tDNA（Ser）GCT基因编码的唯一种类，每个基因组中有三个拷贝，因为未检测到其他相应的表达tRNA。因此，该基因的表达非常低。与极低的细胞内含量相比，颗粒中tRNA（Ser）GCU的高浓度使我们考虑其可能参与Ty特异性过程。
