College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun, China.
Microbiologyopen. 2019 May;8(5):e00712. doi: 10.1002/mbo3.712. Epub 2018 Aug 16.
Astragalus membranaceus is an herbaceous perennial plant, growing to about 2 feet tall, with sprawling stems and alternate leaves about 12-24 leaflets. In total, 24 cross bred (Duroc × Landrace × Yorkshire) piglets weaned at 4 weeks with an average body weight of 10.84 ± 1.86 kg, were divided into four groups and randomly assigned to dietary treatments containing different AMSLF levels (0.00%, 2.50%, 5.00%, and 7.50%). The piglets in the control group (0.00% AMSLF) were fed basal diet and other treatment groups were fed basal diet in addition to 2.50%, 5.00%, and 7.50% pulverized AMSLF. The results indicated that supplementation with AMSLF significantly (p < 0.05) decreased diarrheal incidence in piglets. There was significant difference between treatment in terms of ADFI, ADG and FCR. Both 5.00% and 7.50% treatments significantly increased growth performance. The digestibility of gross energy and dry matter increased (p > 0.05) with increasing AMSLF level. The level of blood IL-2 and TNF-α were significantly affected by AMSLF supplementation with 7.50% AMSLF group having higher (p < 0.05) IL-2 and TNF-α levels than the other treatment groups. The 16SrDNA sequencing results from the four treatments showed that the potentially active bacterial microbial population and diversity in pig cecum were dominated by the phyla Bacteriodetes and Firmicutes regardless of the AMSLF supplementation. The Shannon diversity, PD whole tree diversity indices and Chao analyses exhibited significant variability in species richness across the treatments. The principal coordinates analysis (PCoA) showed significant (p < 0.1) differences between bacterial communities in all treatment groups. Results from the current study suggested that AMSLF supplementation increased composition of bacterial microbiota in pig gut. In conclusion, dietary supplements with AMSLF could potentially be used to prevent diarrheal incidence and improved pig production.
黄芪是一种草本多年生植物，生长到约 2 英尺高，具有蔓延的茎和大约 12-24 小叶的互生叶。共有 24 头 4 周龄断奶的杂交（杜洛克×长白×约克夏）仔猪，平均体重为 10.84±1.86kg，分为四组，并随机分配到含有不同 AMSLF 水平（0.00%、2.50%、5.00%和 7.50%）的饮食处理中。对照组（0.00% AMSLF）仔猪饲喂基础日粮，其他处理组在基础日粮中添加 2.50%、5.00%和 7.50%粉碎的 AMSLF。结果表明，添加 AMSLF 可显著（p<0.05）降低仔猪腹泻发生率。在 ADFI、ADG 和 FCR 方面，处理之间存在显著差异。5.00%和 7.50%的处理均显著提高了生长性能。随着 AMSLF 水平的增加，总能和干物质的消化率增加（p>0.05）。血液中 IL-2 和 TNF-α 的水平受到 AMSLF 补充的显著影响，7.50% AMSLF 组的 IL-2 和 TNF-α 水平显著高于其他处理组（p<0.05）。来自四个处理组的 16SrDNA 测序结果表明，无论是否添加 AMSLF，猪盲肠中潜在的活性细菌微生物种群和多样性都以厚壁菌门和拟杆菌门为主。Shannon 多样性、PD 全树多样性指数和 Chao 分析在各处理间表现出物种丰富度的显著变化。主坐标分析（PCoA）显示了所有处理组细菌群落之间的显著差异（p<0.1）。本研究结果表明，添加 AMSLF 可增加猪肠道细菌微生物群的组成。总之，添加 AMSLF 的膳食补充剂可能有助于预防仔猪腹泻的发生，提高仔猪的生产性能。
