School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, 310027, China.
The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.
J Mater Sci Mater Med. 2018 Aug 17;29(9):136. doi: 10.1007/s10856-018-6141-6.
Surface nanotopography and bioactive ions have been considered to play critical roles on the interactions of biomaterials with cells. In this study, a TiO nanorod film incorporated with Zn-containing bioactive glass (TiO/Zn-BG) was prepared on tantalum substrate, trying to evaluate the synergistic effects of nanotopograpgy and bioactive ions to promote cellular osteogenic differentiation activity. The expression of osteogenic-related genes, ALP as well as the ECM mineralization on TiO/Zn-BG film were significantly upregulated compared to that of the film without TiO nanorod nanostructure (Zn-BG) or without Zn (TiO/BG). Moreover, a much low Zn release level on TiO/Zn-BG film was beneficial to promote the osteogenesis, which could be ascribed to that a semi-closed space established by TiO nanorods with adhered cells provided an appropriate micro-environment that facilitated Zn adsorption.
表面纳米形貌和生物活性离子被认为在生物材料与细胞的相互作用中起着关键作用。在这项研究中，在钽基底上制备了掺入含 Zn 生物活性玻璃（TiO/Zn-BG）的 TiO 纳米棒薄膜，试图评估纳米形貌和生物活性离子的协同作用对促进细胞成骨分化活性的影响。与没有 TiO 纳米棒纳米结构（Zn-BG）或没有 Zn（TiO/BG）的薄膜相比，TiO/Zn-BG 薄膜上的成骨相关基因表达、ALP 以及 ECM 矿化明显上调。此外，TiO/Zn-BG 薄膜上较低的 Zn 释放水平有利于促进成骨，这可能是由于附着在细胞上的 TiO 纳米棒形成的半封闭空间提供了一个合适的微环境，有利于 Zn 的吸附。
