School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, 310027, China.
School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, 310027, China; The Affiliated Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.
Colloids Surf B Biointerfaces. 2018 Jul 1;167:213-219. doi: 10.1016/j.colsurfb.2018.04.012. Epub 2018 Apr 5.
Titanium and tantalum have been widely used for orthopedic and dental implant applications. However, how their inherent surface features regulate cellular osteogeneses still remains elusive. In this study, we engineered two distinct TiO and TaO nanorod films as the two model oxidized surfaces to investigate their intrinsic osteogenic behaviors. The results indicated that the distinctive gradient on zeta potential against pH, corresponding to the deprotonation rate, but not the hydroxyl amount or hydroxylation polarity played a critical role on the cellular osteogenic performance. TiO nanorod film with a higher deprotonation rate significantly upregulated the expression of osteogeneses-related gene and protein, comparing to that of TaO nanorod film. These results might be attributed to that surface with higher deprotonation rateprovided more Bronsted acid-base surface sites to react with protein residues, leading to a mild change in conformation of the absorbed proteins, and subsequently facilitating to trigger the integrin-focal adhesion cytoskeleton actin transduction pathway. This study, therefore, provides a new insight into the understanding the role of material surface hydroxylation on cellular osteogenic responses.
钛和钽已广泛应用于骨科和牙科植入物。然而，它们固有的表面特征如何调节细胞成骨仍然难以捉摸。在这项研究中，我们设计了两种不同的 TiO 和 TaO 纳米棒薄膜作为两种模型氧化表面，以研究它们内在的成骨行为。结果表明，zeta 电位随 pH 值的变化所对应的去质子化速率，而不是羟基数量或羟基极性，对细胞成骨性能起着关键作用。具有更高去质子化速率的 TiO 纳米棒薄膜显著上调了成骨相关基因和蛋白的表达，与 TaO 纳米棒薄膜相比。这可能是由于具有更高去质子化速率的表面提供了更多的布朗斯特酸碱表面位点与蛋白质残基反应，导致吸附蛋白质构象发生轻微变化，进而促进整合素-粘着斑细胞骨架肌动蛋白转导途径的激活。因此，本研究为理解材料表面羟基化对细胞成骨反应的作用提供了新的见解。
