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具有激酶活性的人巨细胞病毒病毒体相关蛋白。

Human cytomegalovirus virion-associated protein with kinase activity.

作者信息

Britt W J, Auger D

出版信息

J Virol. 1986 Jul;59(1):185-8. doi: 10.1128/JVI.59.1.185-188.1986.

DOI:10.1128/JVI.59.1.185-188.1986
PMID:3012117
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253057/
Abstract

Protein kinase activity was detected in immunoprecipitates of human cytomegalovirus virions and infected cells by using a monoclonal antibody directed against an abundant 68,000-dalton virion structural protein. Purification of this protein by electrophoresis confirmed that the kinase activity was associated with this protein. The kinase activity was dependent on divalent cations (Mg2+, Mn2+) and cyclic nucleotide independent and exhibited optimal activity at pH 7 to 8. The kinase phosphorylated threonine and serine but not tyrosine.

摘要

通过使用针对一种丰富的68000道尔顿病毒体结构蛋白的单克隆抗体，在人巨细胞病毒病毒体和感染细胞的免疫沉淀物中检测到蛋白激酶活性。通过电泳纯化该蛋白证实激酶活性与该蛋白相关。激酶活性依赖于二价阳离子（Mg2+、Mn2+），不依赖环核苷酸，在pH 7至8时表现出最佳活性。该激酶使苏氨酸和丝氨酸磷酸化，但不使酪氨酸磷酸化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/f7f005e33073/jvirol00106-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/ad5023a1849b/jvirol00106-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/30536d264b74/jvirol00106-0194-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/f7f005e33073/jvirol00106-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/ad5023a1849b/jvirol00106-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/30536d264b74/jvirol00106-0194-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/253057/f7f005e33073/jvirol00106-0195-a.jpg

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本文引用的文献

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Human cytomegalovirus-associated DNA polymerase and protein kinase activities.人巨细胞病毒相关的DNA聚合酶和蛋白激酶活性
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