Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran.
Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Science, Tabriz, Iran.
Carbohydr Polym. 2019 Mar 15;208:294-301. doi: 10.1016/j.carbpol.2018.12.066. Epub 2018 Dec 21.
The aim of present work is to improve the solubility, surface charged and capacity of drug loading of graphene oxide (GO) by modification of GO with carboxymethylcellulose (CMC) and Zinc-based metal-organic framework (MOF-5) to realize and control accurately the release manner. To achieve this aim, carboxymethylcellulose/Zinc-based metal-organic framework/graphene oxide bio-nanocomposite (CMC/MOF-5/GO) as a new drug delivery system was synthesized in one-pot through the solvothermal technique. The prepared CMC/MOF-5/GO was characterized and used as a carrier to encapsulate the doxorubicin (DOX) as an anticancer drug. The obtained compounds were characterized using SEM, AFM, XRD, FTIR, EDX spectroscopy, BET and Zeta potentials-DLS analysis. The AFM images of GO and CMC/MOF-5/GO illustrated that the sheet thickness of GO was around 30 nm, which increased to ˜80 nm after modification with CMC and MOF-5.In addition, the drug delivery evaluation showed that the DOX-loaded bio-nanocomposites enhanced anticancer properties. Under tumor cell microenvironment at pH 5, the DOX release rate was significantly higher than that under physiological conditions at pH 7.4. The MTT results showed that DOX@CMC/MOF-5/GO exhibits notable cytotoxicity to K562 cells. The resulted bio-nanocomposite showed that this carrier system could be potentially used in anticancer drug delivery systems.
本工作旨在通过羧甲基纤维素（CMC）和锌基金属有机骨架（MOF-5）对氧化石墨烯（GO）进行改性，提高其溶解度、表面荷电能力和载药量，从而实现并精确控制药物的释放方式。为了实现这一目标，通过溶剂热技术，在一锅法中合成了羧甲基纤维素/锌基金属有机骨架/氧化石墨烯生物纳米复合材料（CMC/MOF-5/GO）作为新型药物传递系统。对制备的 CMC/MOF-5/GO 进行了表征，并用作载体包封阿霉素（DOX）作为抗癌药物。采用 SEM、AFM、XRD、FTIR、EDX 光谱、BET 和 Zeta 电位-DLS 分析对所得化合物进行了表征。GO 和 CMC/MOF-5/GO 的 AFM 图像表明，GO 的片层厚度约为 30nm，经 CMC 和 MOF-5 改性后增加到约 80nm。此外，药物输送评估表明，载药生物纳米复合材料增强了抗癌性能。在 pH5 的肿瘤细胞微环境下，DOX 的释放速率明显高于 pH7.4 的生理条件下的释放速率。MTT 结果表明，DOX@CMC/MOF-5/GO 对 K562 细胞具有显著的细胞毒性。所得的生物纳米复合材料表明，该载体系统可潜在用于抗癌药物传递系统。
