Nie Wei, Wang Bo, Gao Jing, Guo Yuming, Wang Zhong
1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
2Department of Animal Science, Washington State University, Pullman, Washington USA.
J Anim Sci Biotechnol. 2018 Aug 13;9:53. doi: 10.1186/s40104-018-0271-z. eCollection 2018.
Phosphorus is an essential nutrient to maintain poultry health and performance. The objective of this study was to evaluate the effect of dietary phosphorus levels on egg production, egg quality, bone health, immune responses of laying hens challenged with lipopolysaccharide.
Three hundred laying hens at 28 wk were randomly divided into 2 dietary treatments with 10 replicates of 15 birds. The wheat-soybean based diets contained either 0.12% or 0.4% non-phytate phosphorus (NPP). At 32 wk of age, all the birds of each dietary treatment were injected into the abdomen with 1.5 mg/kg body weight (BW) of either LPS or saline once a day at 24-h intervals for continuous 9 d. The performance of laying hens was evaluated for 9 d. The eggs after the fifth injection were collected to value the egg quality. Three hours after the first injection, blood was collected to measure serum metabolite and immune response associated parameters. Three hours after the fifth injection, the hens were euthanized to obtain tibia, cecal tonsils and jejunum.
Compared with saline-injected hens, LPS-injected hens had lower feed intake and egg production ( < 0.05). Eggshell thickness, strength, albumin height and Haugh unit were significantly increased in LPS-injected hens compared with saline-injected hens ( < 0.05). Furthermore, laying hens challenged with LPS had lower villious height/ crypt depth ration than those received saline. Serum calcium, phosphorus and SOD activities significantly decreased in the LPS-injected hens compared with the control ( < 0.05). LPS up-regulated expression of , and in cecum, and serum concentration of MDA, IL-1β and IL-6 ( < 0.05), whereas 0.40% dietary non-phytate phosphorus supplementation significantly increased ( < 0.05) villi height/crypt depth ratio, decreased ( < 0.05) serum MDA and IFN-γ concentration compared with the 0.12% non-phytate phosphorus group.
In summary, this study demonstrates that 0.40% dietary non-phytate phosphorus supplementation significantly increased calcium and phosphorus levels of eggshell, increased villi height/crypt depth ratio, decreased serum MDA and IFN-γ concentration compared with the 0.12% non-phytate phosphorus groups. The results indicate that high level of dietary non-phytate phosphorus exerts a potential effect in alleviating systemic inflammation of LPS-challenged laying hens.
磷是维持家禽健康和生产性能所必需的营养素。本研究的目的是评估日粮磷水平对产蛋量、蛋品质、骨骼健康以及脂多糖攻击的蛋鸡免疫反应的影响。
将28周龄的300只蛋鸡随机分为2种日粮处理组，每组10个重复，每个重复15只鸡。以小麦-大豆为基础的日粮分别含有0.12%或0.4%的非植酸磷（NPP）。在32周龄时，对每种日粮处理组的所有鸡每天按24小时间隔腹腔注射1.5mg/kg体重的脂多糖或生理盐水，持续9天。对蛋鸡的生产性能进行9天的评估。在第五次注射后收集鸡蛋以评估蛋品质。在第一次注射后3小时，采集血液以测量血清代谢物和免疫反应相关参数。在第五次注射后3小时，对母鸡实施安乐死以获取胫骨、盲肠扁桃体和空肠。
与注射生理盐水的母鸡相比，注射脂多糖的母鸡采食量和产蛋量较低（P<0.05）。与注射生理盐水的母鸡相比，注射脂多糖的母鸡蛋壳厚度、强度、蛋白高度和哈氏单位显著增加（P<0.05）。此外，受到脂多糖攻击的蛋鸡绒毛高度/隐窝深度比值低于注射生理盐水的蛋鸡。与对照组相比，注射脂多糖的母鸡血清钙、磷和超氧化物歧化酶活性显著降低（P<0.05）。脂多糖上调了盲肠中[相关基因名称未给出]、[相关基因名称未给出]和[相关基因名称未给出]的表达，以及血清丙二醛、白细胞介素-1β和白细胞介素-6的浓度（P<0.05），而与0.12%非植酸磷组相比，日粮添加0.
