Department of Animal Sciences, Purdue University, West Lafayette, IN 47906, USA
Poult Sci. 2010 Jan;89(1):28-33. doi: 10.3382/ps.2009-00464.
The acute phase response (APR) is characterized by inflammation, fever, and altered organ metabolism resulting in muscle catabolism and anorexia. Lipopolysaccharide (LPS)-induced APR may reflect depressed growth and appetite loss. Therefore, a 1-wk growth experiment was conducted to examine whether dietary supplementation of a multispecies probiotic (PoultryStar) would alleviate growth suppression and anorexia caused by LPS-induced APR. The experiment was designed with 4 treatments (n = 8 cages/treatment; 6 birds/cage) starting at 14 d of age. Before (0 to 14 d of age) and for the experiment (14 to 21 d of age), male broiler chicks were fed diets devoid of probiotic or were supplemented with 1.7 x 10(8) cfu/kg of probiotic. At 14 d of age, birds fed the diet devoid of probiotic were further divided into 3 treatments: an unchallenged positive control, LPS-challenged negative control (LPS-NC), and a treatment that was pair-fed to LPS-NC. The probiotic-fed birds were also then challenged with LPS. The LPS (Escherichia coli 055:B5) was injected intraperitoneally 4 times at 48-h intervals at 1 mg/kg of BW. The LPS challenge dramatically depressed BW gain from 14 to 21 d of age by 22% (P < 0.001). However, 41% of growth depression was attributable to factors other than feed intake reduction when compared with the pair-fed treatment. Probiotic supplementation recovered 17% of depressed growth (vs. LPS-NC; P = 0.068), but this improved growth was not due to improvements in feed intake (P = 0.47). However, recovery of feed intake of the probiotic + LPS birds occurred 48-h earlier than the LPS-NC birds. Growth depression induced by LPS administration resulted in an overall relative feed intake (vs. positive control) of 0.83 and also decreased net energy and protein accretion. Probiotic supplementation did not alleviate the reduction in net energy or protein accretion induced by LPS. In conclusion, APR (induced by LPS administration) diverted a large portion of consumed nutrients from tissue accretion. Probiotic supplementation lessened the anorexic effects of LPS resulting in a trend toward BW gain improvement versus the LPS-NC.
急性期反应 (APR) 的特征为炎症、发热和器官代谢改变，导致肌肉分解和厌食。脂多糖 (LPS) 诱导的 APR 可能反映出生长抑制和食欲下降。因此，进行了为期 1 周的生长实验，以检验日粮中添加多物种益生菌 (PoultryStar) 是否可以缓解 LPS 诱导的 APR 引起的生长抑制和厌食。实验设计了 4 种处理 (n = 8 个笼/处理；每个笼 6 只鸡)，从 14 日龄开始。在 0-14 日龄和实验期间 (14-21 日龄)，雄性肉鸡雏鸡饲喂不含益生菌的日粮或添加 1.7×10(8)cfu/kg 的益生菌。在 14 日龄时，饲喂不含益生菌日粮的雏鸡进一步分为 3 种处理：未受挑战的阳性对照组、LPS 挑战的阴性对照组 (LPS-NC) 和 LPS-NC 的配对饲喂组。饲喂益生菌的雏鸡也接受 LPS 挑战。LPS (大肠杆菌 055:B5) 以 1 mg/kg BW 的剂量腹腔内注射 4 次，间隔 48 小时。LPS 挑战导致 14-21 日龄体重增加减少了 22% (P < 0.001)。然而，与配对饲喂组相比，41%的生长抑制归因于采食量降低以外的因素。益生菌补充恢复了 17%的生长抑制 (与 LPS-NC 相比；P = 0.068)，但这种生长改善不是由于采食量的提高 (P = 0.47)。然而，益生菌+LPS 组的采食量恢复比 LPS-NC 组早 48 小时。LPS 给药引起的生长抑制导致相对采食量 (与阳性对照组相比) 总体上为 0.83，并降低了净能和蛋白质沉积。益生菌补充没有减轻 LPS 引起的净能或蛋白质沉积减少。总之，APR (由 LPS 给药引起) 将消耗的大部分营养素从组织沉积中转移出来。益生菌补充减轻了 LPS 的厌食作用，与 LPS-NC 相比，体重增加趋势有所改善。
