Zolotova N A, Polikarpova A V, Khochanskii D N, Makarova O V, Mikhailova L P
Research Institute of Human Morphology, Moscow, Russia.
Bull Exp Biol Med. 2018 Aug;165(4):434-437. doi: 10.1007/s10517-018-4187-6. Epub 2018 Aug 20.
We studied changes in the expression of mRNA for mucins and claudins in the medial part of the colon in male C57Bl/6 mice on the model of acute and chronic colitis induced by substitution of drinking water with 1% solution of dextran sodium sulphate for 5 days. In acute colitis, the expression of the main structural component of glycocalyx, mucin Muc3, decreased and expression of pore-forming claudin Cldn2 increased, which reflected enhanced permeability of tight junctions. In the chronic colitis group, in comparison with the normal group, we observed an increase in expression of mRNA of main structural mucus component Muc2, enhanced of expression of Muc1 associated with carcinogenesis, and reduced expression of Muc13, which led to a more severe course of colitis; the expression of pore-forming claudin Cldn2 was elevated. These findings indicate that the imbalance in the expression of mucins and claudins plays an important role in the mechanisms of development of acute and chronic colitis.
我们研究了在以1%硫酸葡聚糖钠溶液替代饮用水5天诱导的急性和慢性结肠炎模型中，雄性C57Bl/6小鼠结肠内侧粘蛋白和闭合蛋白mRNA表达的变化。在急性结肠炎中，糖萼的主要结构成分粘蛋白Muc3的表达降低，而形成孔道的闭合蛋白Cldn2的表达增加，这反映了紧密连接通透性增强。在慢性结肠炎组中，与正常组相比，我们观察到主要结构性粘液成分Muc2的mRNA表达增加，与致癌作用相关的Muc1表达增强，以及Muc13表达降低，这导致结肠炎病情更严重；形成孔道的闭合蛋白Cldn2的表达升高。这些发现表明，粘蛋白和闭合蛋白表达的失衡在急性和慢性结肠炎的发生机制中起重要作用。
