来自大鼠中性粒细胞裂解物的5-脂氧合酶。
5-lipoxygenase from rat PMN lysate.
作者信息
Skoog M T, Nichols J S, Wiseman J S
出版信息
Prostaglandins. 1986 Mar;31(3):561-76. doi: 10.1016/0090-6980(86)90117-6.
The products of arachidonic acid metabolism in the 15,000xg supernatant of sonicated rat PMN are described. Only products derived from 5-lipoxygenase are observed. These products are 5-HETE and products derived from hydrolysis of LTA4, particularly LTB4. Some minor products derived from decomposition of 5-HPETE are also observed. The dependence of the activity of 5-lipoxygenase on enzyme and on substrate concentrations is presented and discussed in terms of a kinetic model that includes enzyme inactivation during turnover and substrate inhibition. The 5-lipoxygenase activity is stimulated by Ca++ and ATP.
本文描述了超声处理的大鼠多形核白细胞（PMN）15,000xg 上清液中花生四烯酸代谢产物。仅观察到源自 5-脂氧合酶的产物。这些产物是 5-羟基二十碳四烯酸（5-HETE）以及源自白三烯 A4（LTA4）水解的产物，特别是白三烯 B4（LTB4）。还观察到一些源自 5-氢过氧二十碳四烯酸（5-HPETE）分解的次要产物。根据一个动力学模型，阐述并讨论了 5-脂氧合酶活性对酶和底物浓度的依赖性，该模型包括周转过程中的酶失活和底物抑制。5-脂氧合酶活性受到钙离子（Ca++）和三磷酸腺苷（ATP）的刺激。
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