Lemos Natália Emerim, Farias Mariela Granero, Kubaski Francyne, Scotti Luciana, Onsten Tor Gunnar Hugo, Brondani Letícia de Almeida, Wagner Sandrine Comparsi, Sekine Leo
Universidade Feevale, Novo Hamburgo, RS, Brazil; Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
Hematol Transfus Cell Ther. 2018 Jul-Sep;40(3):213-218. doi: 10.1016/j.htct.2018.01.002. Epub 2018 Mar 15.
Due to laboratory logistic issues, our center has traditionally scheduled peripheral blood stem cell harvests based on timing from the start of mobilization. This has proved to be useful in some cases, but also resulted in many fruitless harvests due to poor mobilization. In order to improve the efficiency of collections and compare the effectiveness of peripheral blood CD34 cells as a predictor with data from other reports, this study analyzed the implementation of this routine.
Peripheral blood and leukapheresis samples were quantified by flow cytometry and the association between these parameters was assessed.
Sixty-six consecutive leukapheresis samples were collected from 34 patients after the collection of peripheral blood samples for CD34 quantification. A moderate positive correlation was observed between peripheral blood CD34 cell count and total CD34 cell count/kg ( = 0.596; -value < 0.001). A multivariable regression model also confirmed this association and allowed the estimation that for every increase in five CD34 cells/μL in the peripheral blood, a mean increase of 0.38 × 10 CD34 cells/kg could be predicted. Demographic characteristics, baseline comorbidities and mobilization regimen did not influence final CD34 cell count in this sample.
As observed in other centers, quantification of peripheral blood CD34 progenitor cells is a strong predictor of effectiveness to guide stem cell harvesting. Due to the results of this study, a modification in the peripheral blood stem cell harvesting logistics was implemented at our center in order to incorporate this routine.
由于实验室后勤问题，我们中心传统上根据动员开始后的时间安排外周血干细胞采集。这在某些情况下已证明是有用的，但也因动员效果不佳导致许多采集无果而终。为了提高采集效率，并将外周血CD34细胞作为预测指标的有效性与其他报告的数据进行比较，本研究分析了该常规做法的实施情况。
通过流式细胞术对外周血和白细胞分离样本进行定量，并评估这些参数之间的关联。
在采集用于CD34定量的外周血样本后，从34例患者中连续采集了66份白细胞分离样本。外周血CD34细胞计数与每千克总CD34细胞计数之间观察到中度正相关（r = 0.596；P值<0.001）。多变量回归模型也证实了这种关联，并估计外周血中每增加5个CD34细胞/μL，可预测每千克平均增加0.38×10⁶个CD34细胞。人口统计学特征、基线合并症和动员方案在该样本中均未影响最终的CD34细胞计数。
正如在其他中心所观察到的，外周血CD34祖细胞的定量是指导干细胞采集有效性的有力预测指标。基于本研究结果，我们中心对外周血干细胞采集后勤工作进行了调整，以纳入该常规做法。
