牛痘病毒、单纯疱疹病毒和致癌物可在一种人类细胞系中诱导DNA扩增，并支持一种依赖辅助病毒的细小病毒的复制。

Vaccinia virus, herpes simplex virus, and carcinogens induce DNA amplification in a human cell line and support replication of a helpervirus dependent parvovirus.

作者信息

Schlehofer J R, Ehrbar M, zur Hausen H

出版信息

Virology. 1986 Jul 15;152(1):110-7. doi: 10.1016/0042-6822(86)90376-4.

Abstract

The SV40-transformed human kidney cell line, NB-E, amplifies integrated as well as episomal SV40 DNA upon treatment with chemical (DMBA) or physical (uv irradiation) carcinogens ("initiators") as well as after infection with herpes simplex virus (HSV) type 1 or with vaccinia virus. In addition it is shown that vaccinia virus induces SV40 DNA amplification also in the SV40-transformed Chinese hamster embryo cell line, CO631. These findings demonstrate that human cells similar to Chinese hamster cells amplify integrated DNA sequences after treatment with carcinogens or infection with specific viruses. Furthermore, a poxvirus--vaccinia virus--similar to herpes group viruses induces DNA amplification. As reported for other systems, the vaccinia virus-induced DNA amplification in NB-E cells is inhibited by coinfection with adeno-associated virus (AAV) type 5. This is in line with previous studies on inhibition of carcinogen- or HSV-induced DNA amplification in CO631 cells. The experiments also demonstrate that vaccinia virus, in addition to herpes and adenoviruses acts as a helper virus for replication and structural antigen synthesis of AAV-5 in NB-E cells.

摘要

经猿猴空泡病毒40（SV40）转化的人肾细胞系NB-E，在用化学致癌物（二甲基苯并蒽，DMBA）或物理致癌物（紫外线照射）处理后，以及在感染1型单纯疱疹病毒（HSV）或痘苗病毒后，会扩增整合型以及游离型SV40 DNA。此外，研究表明痘苗病毒在经SV40转化的中国仓鼠胚胎细胞系CO631中也能诱导SV40 DNA扩增。这些发现表明，与中国仓鼠细胞类似，人细胞在用致癌物处理或感染特定病毒后会扩增整合的DNA序列。此外，一种痘病毒——痘苗病毒——与疱疹病毒群病毒类似，能诱导DNA扩增。正如其他系统所报道的，在NB-E细胞中，痘苗病毒诱导的DNA扩增会被5型腺相关病毒（AAV）共感染所抑制。这与之前关于CO631细胞中致癌物或HSV诱导的DNA扩增抑制的研究结果一致。实验还表明，痘苗病毒除了疱疹病毒和腺病毒外，还作为辅助病毒促进NB-E细胞中AAV-5的复制和结构抗原合成。