[基于微/纳米结构与抗菌肽协同作用的钛金属表面抗菌性能研究]
[Study on antibacterial properties of titanium metallic surface due to synergistic action of micro/nano-structure and antimicrobial peptides].
作者信息
He Hongyan, Gao Pengyang, Qiao Zhongqian, Qu Xue, Liu Changsheng
机构信息
Medical Biomaterials Engineering Research Center of the Ministry of Education, East China University of Science and Technology, Shanghai, 200237, P.R.China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, P.R.China.
Medical Biomaterials Engineering Research Center of the Ministry of Education, East China University of Science and Technology, Shanghai, 200237, P.R.China.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Sep 15;32(9):1116-1122. doi: 10.7507/1002-1892.201805022.
OBJECTIVE
To investigate the effects of micro/nano-structure and antimicrobial peptides (AMPs) on antibacterial properties for titanium (Ti) metal surface.
METHODS
Ti disks were treated via sandblasted large-grit acid-etched (SLA) and alkali-heat treatment (AHT) to build the micor/nano-structure, on which AMPs were spin-coated with a certain amount (10, 30, 50, 70, and 90 μg). Scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) were used to observe the surface structure and characterize the surface elements (i.e. contents of C, N, O, and Ti). Ti disks loaded with AMPs of difference amounts were co-cultured with ( ) for 24 hours. After that, the formation and dimension of antibacterial circle were measured. Furthermore, the Ti disks treated with different approaches (untreated, SLA treatment, SLA+THA treatment, and loaded with 90 μg AMPs) were co-cultured with and ( ) for 3 hours, bacterial adhesion on the disks were evaluated by using SEM. The antibacterial performances in solution were quantitatively evaluated by immersing the Ti disks in bacterial solutions and measuring the absorbance ( ) values.
RESULTS
It was found that the nanoporous structure could be easily constructed by SLA+AHT approach. After spin-coating AMPs, the nanopores with the diameter less than 200 nm were almost covered. According to the element analysis, with the increase of AMPs, the C content gradually increased; the N content was not detected until AMPs amount reached 70 μg on the disks. The diameter of antibacterial circle clearly depended on the AMPs amount. The Ti disks loaded with 90 μg AMPs had significantly larger antibacterial circles than the other Ti disks ( <0.05). Based on the SEM observation, the Ti disks loaded with 90 μg AMPs has the least bacterial attachment compared with the other Ti disks ( <0.05). The value of bacterial solution immersed with the Ti disks loaded with 90 μg AMPs was much lower than the other Ti disks ( <0.05).
CONCLUSION
The approach of micro/nano-structure and AMPs can improve the antibacterial properties of Ti metal surface.
目的
研究微/纳米结构及抗菌肽（AMPs）对钛（Ti）金属表面抗菌性能的影响。
方法
通过喷砂大颗粒酸蚀（SLA）和碱热处理（AHT）对钛盘进行处理以构建微/纳米结构，然后在其上旋涂一定量（10、30、50、70和90μg）的抗菌肽。使用扫描电子显微镜（SEM）和能谱仪（EDS）观察表面结构并表征表面元素（即C、N、O和Ti的含量）。将负载不同量抗菌肽的钛盘与（ ）共培养24小时。之后，测量抑菌圈的形成和尺寸。此外，将采用不同处理方法（未处理、SLA处理、SLA+THA处理以及负载90μg抗菌肽）的钛盘与（ ）和（ ）共培养3小时，通过SEM评估钛盘上的细菌粘附情况。通过将钛盘浸入细菌溶液中并测量吸光度（ ）值来定量评估溶液中的抗菌性能。
结果
发现通过SLA+AHT方法可轻松构建纳米多孔结构。旋涂抗菌肽后，直径小于200nm的纳米孔几乎被覆盖。根据元素分析，随着抗菌肽含量的增加，C含量逐渐增加；直到钛盘上抗菌肽含量达到70μg时才检测到N含量。抑菌圈的直径明显取决于抗菌肽的含量。负载90μg抗菌肽的钛盘的抑菌圈明显大于其他钛盘（<0.05）。基于SEM观察，与其他钛盘相比，负载90μg抗菌肽的钛盘上的细菌附着最少（<0.05）。负载90μg抗菌肽的钛盘浸泡的细菌溶液的（ ）值远低于其他钛盘（<0.05）。
结论
微/纳米结构与抗菌肽相结合的方法可提高钛金属表面的抗菌性能。
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