Song Tianxi, Hu Yanli, Cui Yun, He Zhimin, Zhu Jinliang, Wang Xiumei, Qiu Zhiye
Beijing Allgens Medical Technology Co., Ltd., Beijing, 100176, P.R.China.
School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, P.R.China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Sep 15;32(9):1137-1143. doi: 10.7507/1002-1892.201804119.
To investigate the bone repair and regeneration ability of biomimetic mineralized collagen bone graft material and autologous bone marrow in rabbit posterolateral spinal fusion model.
Twenty-seven 20-week-old male New Zealand white rabbits ［weighing (5.0±0.5) kg］ were used to establish the posterolateral spinal fusion model of L and L segments by stripping the transverse process and exposing cancellous bone with electric burr. The rabbits were randomly divided into 3 groups, 9 in each group. Groups A, B, and C were implanted 1.5 mL autologous iliac bone, 1.5 mL (30 mm×10 mm×5 mm) biomimetic mineralized collagen bone graft material, and 1.5 mL (30 mm×10 mm×5 mm) biomimetic mineralized collagen bone graft material and autologous bone marrow in each bone defect. At 4, 8, and 12 weeks after operation, the apparent hardness of the bone grafting area was observed by manipulation method, in order to evaluate bone graft fusion effects. Three animals were sacrificed in each group at each time point, the vertebral body specimens were excised and the bone defect repair and fusion were observed by X-ray films, and three-dimensional CT examination was performed to evaluate whether new bone was formed in the body. HE staining was performed at each time point to observe the formation of new bone and the repair and fusion of bone defects.
The manipulation test showed that bone graft fusion was not found in all groups at 4 weeks after operation; 3 (50.0%), 2 (33.3%), and 4 (66.7%) of groups A, B, and C reached bone graft fusion at 8 weeks after operation; 5 (83.3%), 4 (66.7%), and 5 (83.3%) of groups A, B, and C reached bone graft fusion at 12 weeks after operation; the fusion rate of group C was similar to that of group A, and all higher than that of group B. X-ray film observation showed that the fusion rate of group C at 8 and 12 weeks after operation was higher than that of group B, similar to group A. Three-dimensional CT observation showed that the effect of bone fusion in group C was better than that in group B, which was close to group A. HE staining observation showed that large area of mature lamellar bone coverage appeared in the bone graft area of groups A, B, and C at 12 weeks after operation, the material was completely degraded, and the marginal boundary of the host bone disappeared and tightly combined.
Biomimetic mineralized collagen bone graft material mixed with autologous bone marrow has good osteoinduction and osteogenesis guidance. Compared with biomimetic mineralized collagen bone graft material, it has better and faster osteogenesis effect, which is close to autologous bone transplantation.
在兔后外侧脊柱融合模型中研究仿生矿化胶原骨移植材料与自体骨髓的骨修复及再生能力。
选用27只20周龄雄性新西兰白兔［体重(5.0±0.5)kg］，通过剥离横突并用电动磨钻暴露松质骨，建立L和L节段的后外侧脊柱融合模型。将兔子随机分为3组，每组9只。A、B、C组分别在每个骨缺损处植入1.5 mL自体髂骨、1.5 mL(30 mm×10 mm×5 mm)仿生矿化胶原骨移植材料、1.5 mL(30 mm×10 mm×5 mm)仿生矿化胶原骨移植材料与自体骨髓。术后4、8、12周，通过手法触诊观察植骨区的表观硬度，以评估植骨融合效果。每个时间点每组处死3只动物，切取椎体标本，通过X线片观察骨缺损修复及融合情况，并进行三维CT检查以评估体内是否形成新骨。每个时间点进行HE染色，观察新骨形成及骨缺损的修复与融合情况。
手法触诊试验显示，术后4周所有组均未发现植骨融合；术后8周，A、B、C组分别有3只(50.0%)、2只(33.3%)、4只(66.7%)达到植骨融合；术后12周，A、B、C组分别有5只(83.3%)、4只(66.7%)、5只(83.3%)达到植骨融合；C组融合率与A组相似，均高于B组。X线片观察显示，术后8周和12周C组融合率高于B组，与A组相似。三维CT观察显示，C组骨融合效果优于B组，接近A组。HE染色观察显示，术后12周A、B、C组植骨区出现大面积成熟板层骨覆盖，材料完全降解，宿主骨边缘界限消失并紧密结合。
仿生矿化胶原骨移植材料与自体骨髓混合具有良好的骨诱导及成骨引导作用。与仿生矿化胶原骨移植材料相比，其成骨效果更好、更快，接近自体骨移植。
