Department of Gynecology, Affiliated Qilu Hospital of Shandong University, Lixia District, Jinan, Shandong, P.R. China.
Department of Gynecology Ward-3, Linyi People's Hospital, Linyi, Hedong District, Linyi, Shandong, P.R. China.
Oncol Res. 2019 Feb 21;27(3):335-340. doi: 10.3727/096504018X15252202178408. Epub 2018 Aug 21.
Aberrant expression of microRNA-152 (miR-152) is frequently observed in human cancers including ovarian cancer, breast cancer, prostate cancer, and gastric cancer. However, its expression and functional role in cervical cancer (CC) are poorly understood. Also, the association between miR-152 and Krüppel-like factor 5 (KLF5) expression in CC remains unclear. In this study, analyzing the expression of miR-152 by quantitative real-time PCR (qRT-PCR) revealed it was sharply reduced in CC tissues and cell lines. In addition, the negative correlation of miR-152 expression and KLF5 expression was observed. The dual-luciferase reporter assay validated that KLF5 was a target of miR-152. In vitro functional assays revealed that miR-152 could inhibit cell proliferation and cell cycle progression through regulating the expression of KLF5. Taken together, our study suggested that miR-152 functions as a tumor suppressor in CC, and the miR-152/KLF5 axis may provide novel therapeutic targets for CC treatment.
miR-152 的异常表达在包括卵巢癌、乳腺癌、前列腺癌和胃癌在内的多种人类癌症中经常观察到。然而，其在宫颈癌 (CC) 中的表达和功能作用仍不清楚。此外，miR-152 与 Krüppel 样因子 5 (KLF5) 在 CC 中的表达之间的关联尚不清楚。在这项研究中，通过定量实时 PCR (qRT-PCR) 分析 miR-152 的表达发现，它在 CC 组织和细胞系中明显降低。此外，还观察到 miR-152 表达与 KLF5 表达呈负相关。双荧光素酶报告基因实验验证了 KLF5 是 miR-152 的靶基因。体外功能实验表明，miR-152 可以通过调节 KLF5 的表达来抑制细胞增殖和细胞周期进程。总之，我们的研究表明，miR-152 在 CC 中作为一种肿瘤抑制因子发挥作用，miR-152/KLF5 轴可能为 CC 的治疗提供新的治疗靶点。
