Lab of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
Org Biomol Chem. 2018 Aug 29;16(34):6350-6357. doi: 10.1039/c8ob01608a.
Protein persulfidation is a newly defined oxidative posttranslational modification and plays important roles in many biological processes. Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, we developed a novel off-on fluorescent probe for the detection of persulfidation using a chemical sensor, HQO-SSH, in biological systems. HQO-SSH features fast reaction, good selectivity and high sensitivity. Due to the distinctive features of HQO-SSH, this probe was successfully applied to image protein persulfidation changes in pulmonary cells. We also demonstrated that the probe is suitable for imaging protein persulfidation in lung tissues. In addition, confocal imaging with this method revealed that sulfur mustard, a commonly used chemical warfare agent, decreased mitochondrial protein persulfidation in living lung cells and tissues. Due to these results, this probe holds great promise for exploring the role of protein persulfidation in a variety of pathophysiological conditions.
蛋白质过硫化是一种新定义的氧化翻译后修饰，在许多生物过程中发挥着重要作用。为了深入研究 H2S/H2Sn 为基础的信号转导和细胞氧化还原调控，迫切需要在活细胞体系中检测蛋白质过硫化。在此，我们开发了一种新型的基于化学传感器的 HQO-SSH 荧光探针，用于检测生物体系中的过硫化。HQO-SSH 具有快速反应、良好的选择性和高灵敏度的特点。由于 HQO-SSH 的独特性质，该探针成功地应用于肺细胞中蛋白质过硫化变化的成像。我们还证明了该探针适用于肺组织中蛋白质过硫化的成像。此外，利用该方法进行共聚焦成像显示，一种常用的化学战剂芥子气降低了活肺细胞和组织中线粒体蛋白质的过硫化。由于这些结果，该探针有望用于探索蛋白质过硫化在各种病理生理条件下的作用。
