Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Anhui Province Key Laboratory of Major Autoimmune Diseases, Hefei, China.
Department of Rheumatology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China.
J Cell Biochem. 2018 Dec;119(12):10095-10106. doi: 10.1002/jcb.27346. Epub 2018 Aug 21.
The aim of our study was to evaluate two lncRNAs (lnc0640 and lnc5150) expressions and gene single-nucleotide polymorphisms (SNPs) in rheumatoid arthritis (RA) patients.
The expressions of lncRNAs in peripheral blood mononuclear cells (PBMCs) were examined by quantitative real-time reverse transcription polymerase chain reaction from 65 RA patients and 54 controls. Simultaneously, three SNPs (rs13039216, rs6085189, and rs6085190) of lnc0640, three SNPs (rs1590666, rs141561256, and rs144047453) of lnc5150 were genotyped using TaqMan SNP-genotyping assays in 627 RA patients and 590 controls.
The lnc0640 level in PBMCs from RA patients was significantly increased (P = 0.001), whereas the lnc5150 level was significantly reduced (P < 0.001) compared to controls. There were significant associations of lnc0640 and lnc5150 levels with C-reactive protein in RA patients (P = 0.011 and P = 0.014, respectively), while lnc5150 level was associated with erythrocyte sedimentation rate (P = 0.022). TT genotype of rs13039216 in lnc0640 gene was statistically associated with a reduced risk of RA (TT vs CC; P = 0.046), and a decreased risk of rs13039216 variant was observed under the recessive model (P = 0.038). In addition, the G allele of rs141561256 polymorphism in lnc5150 gene was significantly associated with rheumatoid factor in RA patients (P = 0.034). There were no associations between lnc0640 and lnc5150 levels and their respective genotype in RA patients.
The expressions of lnc0640 and lnc5150 were alternated in the RA patients, suggesting that these lncRNAs may involve in the development of RA.
本研究旨在评估类风湿关节炎（RA）患者中两个长链非编码 RNA（lncRNA）（lnc0640 和 lnc5150）的表达和基因单核苷酸多态性（SNP）。
通过定量实时逆转录聚合酶链反应（qRT-PCR）检测 65 例 RA 患者和 54 例对照者外周血单个核细胞（PBMC）中的 lncRNA 表达。同时，在 627 例 RA 患者和 590 例对照者中，采用 TaqMan SNP 基因分型检测方法检测 lnc0640 的三个 SNP（rs13039216、rs6085189 和 rs6085190），以及 lnc5150 的三个 SNP（rs1590666、rs141561256 和 rs144047453）的基因型。
与对照组相比，RA 患者 PBMC 中的 lnc0640 水平显著升高（P=0.001），而 lnc5150 水平显著降低（P<0.001）。lnc0640 和 lnc5150 水平与 RA 患者 C 反应蛋白显著相关（P=0.011 和 P=0.014），而 lnc5150 水平与红细胞沉降率显著相关（P=0.022）。lnc0640 基因 rs13039216 的 TT 基因型与 RA 发病风险降低相关（TT 与 CC；P=0.046），且在隐性模型下观察到 rs13039216 变异的发病风险降低（P=0.038）。此外，lnc5150 基因 rs141561256 多态性的 G 等位基因与 RA 患者的类风湿因子显著相关（P=0.034）。RA 患者中 lnc0640 和 lnc5150 水平及其各自的基因型之间无相关性。
RA 患者 lnc0640 和 lnc5150 的表达发生改变，提示这些 lncRNA 可能参与了 RA 的发病机制。
