Wang Ya-nan, Zhu Xiao-huan, Ma Hui, Du Run-yuan, Li De-rong, Ma Dan-wei
Zhong Yao Cai. 2016 May;39(5):1124-8.
To investigate the inhibition of cell proliferation by essential oil of Chenopodium ambrosioides on the human liver cancer SMMC-7721 cells and human liver LO2 cells,and to study the mechanism of anti-tumor in vitro.
The inhibition of cell proliferation by essential oil of Chenopodium ambrosioides was determined by MTT assay; the distribution of cell cycle was analyzed by flow cytometry( FCM) with PI staining; cell morphology and apoptosis effect of SMMC-7721 cells were observed by microscope; the apoptotic rate was quantified by FCM with Annexin V / PI double staining.
Essential oil of Chenopodium ambrosioides could significantly inhibit the cell proliferation in a concentration-time-dependent manner( P < 0. 05),and the IC50 values on SMMC-7721 cells were lower than human liver LO2 cells at 24,48 and 72 h,respectively( P < 0. 05); cell cycle of SMMC-7721 cells was arrested in G0/G1phase; morphological observation revealed that the cells were wrinkled and the cellular cohesiveness of cells was reduced; nuclear was condensed and in orange colour,of which were the late apoptotic features; and the apoptotic rate increased in a concentration-dependent manner( P < 0. 05),non-viable apoptotic rate was obviously decreased with caspase inhibitor in 100 μg / m L essential oil of Chenopodium ambrosioides( P < 0. 01).
Essential oil of Chenopodium ambrosioides can inhibit SMMC-7721 cell proliferation, which may be related to inducing cell cycle arrest and caspase-dependent apoptosis.
探讨土荆芥挥发油对人肝癌SMMC - 7721细胞和人正常肝LO2细胞增殖的抑制作用，并研究其体外抗肿瘤机制。
采用MTT法检测土荆芥挥发油对细胞增殖的抑制作用；用PI染色，通过流式细胞术（FCM）分析细胞周期分布；用显微镜观察SMMC - 7721细胞的形态及凋亡情况；用Annexin V/PI双染，通过FCM定量凋亡率。
土荆芥挥发油能显著抑制细胞增殖，呈浓度 - 时间依赖性（P < 0.05），在24、48和72 h时，对SMMC - 7721细胞的IC50值均低于人正常肝LO2细胞（P < 0.05）；SMMC - 7721细胞周期阻滞于G0/G1期；形态学观察显示细胞皱缩，细胞间黏附性降低；细胞核固缩，呈橘黄色，为晚期凋亡特征；凋亡率呈浓度依赖性增加（P < 0.05），在100 μg/mL土荆芥挥发油中加入半胱天冬酶抑制剂后，非存活凋亡率明显降低（P < 0.01）。
土荆芥挥发油可抑制SMMC - 7721细胞增殖，其机制可能与诱导细胞周期阻滞及半胱天冬酶依赖性凋亡有关。
