Postgraduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho, São Paulo, SP, Brazil.
Department of Dentistry, Ibirapuera University, São Paulo, SP, Brazil.
PLoS One. 2018 Aug 22;13(8):e0202295. doi: 10.1371/journal.pone.0202295. eCollection 2018.
The aim of this in vitro study, is to evaluate the penetration of a bioceramic root canal sealer into dentinal tubules at 3 mm and 5 mm from the apex after Nd:YAG laser irradiation.
Forty freshly extracted human mandibular premolars were prepared using Reciproc® and irrigated with 17% ethylenediaminetetraacetic acid (EDTA). Teeth were divided into 4 groups: group 1, obturated with control sealer (AH Plus®); group 2, obturated with bioceramic sealer (Endosequence BC Sealer®); group 3, Nd:YAG laser + control sealer (AH Plus®); and group 4, Nd:YAG laser + bioceramic sealer (Endosequence BC Sealer®). The samples were transversely sectioned 3 mm and 5 mm from the apex and examined using confocal laser scanning microscopy. Two parameters were measured: 1) sealer penetration into dentinal tubules of the root canal and 2) sealer penetration into the perimeter of the root canal walls.
Penetration analysis showed that bioceramic sealer had a higher penetration at depths of 3 and 5 mm than that of the control sealer, regardless of laser use (p <0.05). Perimeter analysis showed that there was no difference between both sealers at a depth of 3 mm (p <0.05), regardless of laser use. At a depth of 5 mm, bioceramic sealer and laser showed a greater perimeter of penetration (p <0.05) than the control sealer.
The use of Nd:YAG laser did not compromise the penetration of bioceramic sealer into dentinal tubules of root canals at 3 mm and 5 mm from the apex.
本体外研究旨在评估 Nd:YAG 激光照射后,距根尖 3mm 和 5mm 处牙本质小管内生物陶瓷根管封闭剂的渗透情况。
40 颗新鲜离体下颌前磨牙采用 Reciproc® 根管预备系统预备,并使用 17%乙二胺四乙酸(EDTA)冲洗。将牙齿分为 4 组:第 1 组,用对照封闭剂(AH Plus®)封闭;第 2 组,用生物陶瓷封闭剂(Endosequence BC Sealer®)封闭;第 3 组,Nd:YAG 激光+对照封闭剂(AH Plus®);第 4 组,Nd:YAG 激光+生物陶瓷封闭剂(Endosequence BC Sealer®)。从根尖 3mm 和 5mm 处将样本进行横向切割,并用共聚焦激光扫描显微镜进行检查。测量了两个参数:1)封闭剂在根管牙本质小管中的渗透深度;2)封闭剂在根管壁周界的渗透深度。
渗透分析表明,生物陶瓷封闭剂在 3mm 和 5mm 深度处的渗透深度均高于对照封闭剂,无论是否使用激光(p<0.05)。周界分析表明,在 3mm 深度处,两种封闭剂之间无差异(p<0.05),无论是否使用激光。在 5mm 深度处,生物陶瓷封闭剂和激光显示出比对照封闭剂更大的周界渗透(p<0.05)。
Nd:YAG 激光的使用并未影响生物陶瓷封闭剂在距根尖 3mm 和 5mm 处牙本质小管内的渗透。