Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, 82524 Sohag, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.
Mar Drugs. 2018 Aug 21;16(9):290. doi: 10.3390/md16090290.
A new cyclic hexapeptide, nocardiotide A (), together with three known compounds-tryptophan (), kynurenic acid (), and 4-amino-3-methoxy benzoic acid ()-were isolated and identified from the broth culture of sp. UR67 strain associated with the marine sponge sp. from the Red Sea. The structure elucidation of the isolated compounds were determined based on detailed spectroscopic data including ¹D and ²D nuclear magnetic resonance (NMR) experimental analyses in combination with high resolution electrospray ionization mass spectrometry (HR-ESI-MS), while the absolute stereochemistry of all amino acids components of nocardiotide A () was deduced using Marfey's method. Additionally, ten known metabolites were dereplicated using HR-ESI-MS analysis. Nocardiotide A () displayed significant cytotoxic effects towards the murine CT26 colon carcinoma, human HeLa cervix carcinoma, and human MM.1S multiple myeloma cell lines. The results obtained revealed sponge-associated as a substantial source of lead natural products with pronounced pharmacological activities.
从与红海海绵相关的 sp. UR67 菌株的发酵液中分离得到了一种新的环状六肽诺卡肽 A ()，以及三种已知化合物——色氨酸 (), 犬尿氨酸 (), 和 4-氨基-3-甲氧基苯甲酸 ()。通过详细的光谱数据分析，包括 1D 和 2D 核磁共振 (NMR) 实验分析，结合高分辨率电喷雾电离质谱 (HR-ESI-MS)，确定了分离化合物的结构，而诺卡肽 A ()的所有氨基酸成分的绝对立体化学结构则是使用 Marfey 法推断的。此外，还使用 HR-ESI-MS 分析鉴定了十种已知代谢产物。诺卡肽 A ()对小鼠 CT26 结肠癌细胞、人 HeLa 宫颈癌细胞和人 MM.1S 多发性骨髓瘤细胞系表现出显著的细胞毒性作用。这些结果表明，与海绵相关的 sp. 是具有显著药理活性的先导天然产物的重要来源。
