• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

噬菌体蛋白表达在淋病奈瑟菌的表面，是潜在的疫苗候选物。

Phage proteins are expressed on the surface of Neisseria gonorrhoeae and are potential vaccine candidates.

机构信息

Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.

出版信息

PLoS One. 2018 Aug 23;13(8):e0202437. doi: 10.1371/journal.pone.0202437. eCollection 2018.

DOI:10.1371/journal.pone.0202437
PMID:30138416
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6107182/
Abstract

All Neisseria gonorrhoeae strains whose DNA sequences have been determined possess filamentous phage sequences representing their full genomes. The presence of filamentous phage DNA sequences in all sequenced N. gonorrhoeae strains suggest that purified phage particles might be used as a gonococcal vaccine. To test this hypothesis, we purified filamentous NgoΦfil phages and immunized rabbits subcutaneously. The elicited sera contained large quantities of anti-phage IgG and IgA antibodies that bound to the surface of N. gonorrhoeae cells, as shown by ELISA and flow cytometry. The elicited sera bound to the structural NgoΦ6fil proteins present in phage particles and to N. gonorrhoeae cells. The sera did not react with gonococcal outer membrane proteins. The sera also had bactericidal activity and blocked adhesion of gonococci to tissue culture cells. These data demonstrate that NgoΦfil phage particles can induce antibodies with anti-gonococcal activity and may be a candidate for vaccine development.

摘要

所有已确定 DNA 序列的淋病奈瑟菌菌株都具有代表其全基因组的丝状噬菌体序列。所有测序的淋病奈瑟菌菌株中都存在丝状噬菌体 DNA 序列，这表明纯化的噬菌体颗粒可能被用作淋病球菌疫苗。为了验证这一假设，我们纯化了丝状 NgoΦfil 噬菌体并皮下免疫兔子。ELISA 和流式细胞术显示，诱导的血清含有大量抗噬菌体 IgG 和 IgA 抗体，这些抗体与淋病奈瑟菌细胞表面结合。诱导的血清与噬菌体颗粒和淋病奈瑟菌细胞中存在的结构 NgoΦ6fil 蛋白结合。血清与淋球菌外膜蛋白不反应。血清还具有杀菌活性，并阻止淋球菌黏附到组织培养细胞。这些数据表明，NgoΦfil 噬菌体颗粒可以诱导具有抗淋病活性的抗体，可能是疫苗开发的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/5028af55eb7f/pone.0202437.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/c752cb7065d7/pone.0202437.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/d27ac2cb8fd4/pone.0202437.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/266887f2e821/pone.0202437.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/ddd40d7fa355/pone.0202437.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/7c36b2ebe0ce/pone.0202437.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/c4a7df66a75a/pone.0202437.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/5241c3f1876c/pone.0202437.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/5028af55eb7f/pone.0202437.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/c752cb7065d7/pone.0202437.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/d27ac2cb8fd4/pone.0202437.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/266887f2e821/pone.0202437.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/ddd40d7fa355/pone.0202437.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/7c36b2ebe0ce/pone.0202437.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/c4a7df66a75a/pone.0202437.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/5241c3f1876c/pone.0202437.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c0/6107182/5028af55eb7f/pone.0202437.g008.jpg

相似文献

1
Phage proteins are expressed on the surface of Neisseria gonorrhoeae and are potential vaccine candidates.噬菌体蛋白表达在淋病奈瑟菌的表面，是潜在的疫苗候选物。
PLoS One. 2018 Aug 23;13(8):e0202437. doi: 10.1371/journal.pone.0202437. eCollection 2018.
2
Oral Immunization of Rabbits with S. enterica Typhimurium Expressing Neisseria gonorrhoeae Filamentous Phage Φ6 Induces Bactericidal Antibodies Against N. gonorrhoeae.用表达淋病奈瑟菌丝状噬菌体Φ6的鼠伤寒沙门氏菌对家兔进行口服免疫可诱导产生针对淋病奈瑟菌的杀菌抗体。
Sci Rep. 2016 Mar 4;6:22549. doi: 10.1038/srep22549.
3
Structure of the Recombinant Adhesin Complex Protein (rNg-ACP) and Generation of Murine Antibodies with Bactericidal Activity against Gonococci.重组粘附素复合物蛋白（rNg-ACP）的结构和对淋球菌具有杀菌活性的鼠源抗体的产生。
mSphere. 2018 Oct 10;3(5):e00331-18. doi: 10.1128/mSphere.00331-18.
4
Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage.淋病奈瑟菌基因组中双链DNA原噬菌体序列的特征分析及活性噬菌体的可视化
BMC Microbiol. 2007 Jul 5;7:66. doi: 10.1186/1471-2180-7-66.
5
Gonococcal MtrE and its surface-expressed Loop 2 are immunogenic and elicit bactericidal antibodies.淋球菌 MtrE 及其表面表达的环 2 具有免疫原性，可诱导杀菌抗体。
J Infect. 2018 Sep;77(3):191-204. doi: 10.1016/j.jinf.2018.06.001. Epub 2018 Jun 12.
6
Immunization with recombinant truncated Neisseria meningitidis-Macrophage Infectivity Potentiator (rT-Nm-MIP) protein induces murine antibodies that are cross-reactive and bactericidal for Neisseria gonorrhoeae.用重组截短脑膜炎奈瑟菌-巨噬细胞感染增强蛋白（rT-Nm-MIP）免疫可诱导产生对淋病奈瑟菌具有交叉反应性和杀菌活性的鼠抗体。
Vaccine. 2018 Jun 22;36(27):3926-3936. doi: 10.1016/j.vaccine.2018.05.069. Epub 2018 May 24.
7
The potential for bacteriophages and prophage elements in fighting and preventing the gonorrhea.噬菌体和前噬菌体在防治淋病中的作用潜力。
Crit Rev Microbiol. 2024 Sep;50(5):769-784. doi: 10.1080/1040841X.2023.2274849. Epub 2023 Oct 28.
8
Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.淋病奈瑟菌广谱丝状噬菌体 NgoΦ6 与宿主蛋白的关联。
PLoS One. 2020 Oct 15;15(10):e0240579. doi: 10.1371/journal.pone.0240579. eCollection 2020.
9
Intranasal administration of recombinant Neisseria gonorrhoeae transferrin binding proteins A and B conjugated to the cholera toxin B subunit induces systemic and vaginal antibodies in mice.经鼻给予与霍乱毒素B亚基偶联的重组淋病奈瑟菌转铁蛋白结合蛋白A和B可在小鼠体内诱导产生全身和阴道抗体。
Infect Immun. 2005 Jul;73(7):3945-53. doi: 10.1128/IAI.73.7.3945-3953.2005.
10
Identification of epitopes recognized by monoclonal antibodies SM1 and SM2 which react with all pili of Neisseria gonorrhoeae but which differentiate between two structural classes of pili expressed by Neisseria meningitidis and the distribution of their encoding sequences in the genomes of Neisseria spp.鉴定单克隆抗体SM1和SM2所识别的表位，这两种抗体可与淋病奈瑟菌的所有菌毛发生反应，但能区分脑膜炎奈瑟菌表达的两种菌毛结构类型，以及它们的编码序列在奈瑟菌属基因组中的分布情况。
J Gen Microbiol. 1989 Dec;135(12):3239-51. doi: 10.1099/00221287-135-12-3239.

引用本文的文献

1
Design of respirable sprayed microparticles of encapsulated bacteriophages.包封噬菌体的可吸入喷雾微粒的设计
Front Drug Deliv. 2023 Jun 14;3:1209534. doi: 10.3389/fddev.2023.1209534. eCollection 2023.
2
vaccines: a contemporary overview.疫苗：当代概述。
Clin Microbiol Rev. 2024 Mar 14;37(1):e0009423. doi: 10.1128/cmr.00094-23. Epub 2024 Jan 16.
3
The Optimal Management of Infections.感染的优化管理

本文引用的文献

1
Oral Immunization of Rabbits with S. enterica Typhimurium Expressing Neisseria gonorrhoeae Filamentous Phage Φ6 Induces Bactericidal Antibodies Against N. gonorrhoeae.用表达淋病奈瑟菌丝状噬菌体Φ6的鼠伤寒沙门氏菌对家兔进行口服免疫可诱导产生针对淋病奈瑟菌的杀菌抗体。
Sci Rep. 2016 Mar 4;6:22549. doi: 10.1038/srep22549.
2
Characterization of MDAΦ, a temperate filamentous bacteriophage of Neisseria meningitidis.脑膜炎奈瑟菌温和丝状噬菌体MDAΦ的特性分析
Microbiology (Reading). 2016 Feb;162(2):268-282. doi: 10.1099/mic.0.000215. Epub 2015 Nov 24.
3
Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.
Microorganisms. 2022 Dec 1;10(12):2388. doi: 10.3390/microorganisms10122388.
4
A New Vaccination Method Based on Phage NgoΦ6 and Its Phagemid Derivatives.一种基于噬菌体NgoΦ6及其噬菌粒衍生物的新型疫苗接种方法。
Front Microbiol. 2022 Apr 28;13:793205. doi: 10.3389/fmicb.2022.793205. eCollection 2022.
5
Vaccine Candidates for the Control and Prevention of the Sexually Transmitted Disease Gonorrhea.用于控制和预防性传播疾病淋病的候选疫苗
Vaccines (Basel). 2021 Jul 20;9(7):804. doi: 10.3390/vaccines9070804.
6
Sexually Transmitted Infections-Update on Drug Treatment and Vaccine Development.性传播感染——药物治疗与疫苗研发的最新进展
Medicines (Basel). 2021 Feb 5;8(2):11. doi: 10.3390/medicines8020011.
7
Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.淋病奈瑟菌广谱丝状噬菌体 NgoΦ6 与宿主蛋白的关联。
PLoS One. 2020 Oct 15;15(10):e0240579. doi: 10.1371/journal.pone.0240579. eCollection 2020.
8
Identification of integrative and conjugative elements in pathogenic and commensal species via genomic distributions of DNA uptake sequence dialects.通过 DNA 摄取序列变体在病原和共生种中的基因组分布鉴定整合子和转座子。
Microb Genom. 2020 May;6(5). doi: 10.1099/mgen.0.000372. Epub 2020 May 4.
9
Meningococcal Disease-Associated Prophage-Like Elements Are Present in Neisseria gonorrhoeae and Some Commensal Neisseria Species.淋病奈瑟菌和一些共生奈瑟菌物种中存在脑膜炎奈瑟菌相关类噬菌体元件。
Genome Biol Evol. 2020 Feb 1;12(2):3938-3950. doi: 10.1093/gbe/evaa023.
超越噬菌体展示：丝状噬菌体作为疫苗载体、治疗性生物制品和生物共轭支架的非传统应用。
Front Microbiol. 2015 Aug 4;6:755. doi: 10.3389/fmicb.2015.00755. eCollection 2015.
4
Neisseria gonorrhoeae filamentous phage NgoΦ6 is capable of infecting a variety of Gram-negative bacteria.淋病奈瑟菌丝状噬菌体 NgoΦ6 能够感染多种革兰氏阴性菌。
J Virol. 2014 Jan;88(2):1002-10. doi: 10.1128/JVI.02707-13. Epub 2013 Nov 6.
5
Vaccines against gonorrhea: current status and future challenges.淋病疫苗：现状与未来挑战。
Vaccine. 2014 Mar 20;32(14):1579-87. doi: 10.1016/j.vaccine.2013.08.067. Epub 2013 Sep 6.
6
Beyond gene expression: the impact of protein post-translational modifications in bacteria.超越基因表达：蛋白质翻译后修饰对细菌的影响
J Proteomics. 2014 Jan 31;97:265-86. doi: 10.1016/j.jprot.2013.08.012. Epub 2013 Aug 28.
7
Advances in antiviral vaccine development.抗病毒疫苗研发进展。
Immunol Rev. 2013 Sep;255(1):230-42. doi: 10.1111/imr.12098.
8
Infective and inactivated filamentous phage as carriers for immunogenic peptides.感染性和失活丝状噬菌体作为免疫原性肽的载体。
J Virol Methods. 2012 Jul;183(1):63-8. doi: 10.1016/j.jviromet.2012.03.032. Epub 2012 Apr 3.
9
Clinical applications of DNA vaccines: current progress.DNA 疫苗的临床应用：当前进展。
Clin Infect Dis. 2011 Aug 1;53(3):296-302. doi: 10.1093/cid/cir334.
10
Vaccination with filamentous bacteriophages targeting DEC-205 induces DC maturation and potent anti-tumor T-cell responses in the absence of adjuvants.针对 DEC-205 的丝状噬菌体疫苗接种可在无佐剂的情况下诱导 DC 成熟和有效的抗肿瘤 T 细胞应答。
Eur J Immunol. 2011 Sep;41(9):2573-84. doi: 10.1002/eji.201141526. Epub 2011 Aug 18.