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神经生长因子-硫酸软骨素/羟基磷灰石复合涂层植入物可诱导比格犬种植体周围组织的早期骨整合和神经再生。

Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.

机构信息

Department of Prosthodontics, School and Hospital of Stomatology, Tongji University and Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, People's Republic of China.

State Key Laboratory of Oral Diseases, General Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People's Republic of China.

出版信息

J Orthop Surg Res. 2021 Jan 13;16(1):51. doi: 10.1186/s13018-020-02177-5.

DOI:10.1186/s13018-020-02177-5
PMID:33436038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7805124/
Abstract

BACKGROUND

Osseointegration is the premise of the chewing function of dental implant. Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing. However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.

MATERIALS AND METHODS

NGF-CS/HA-coating composite implants were prepared using the modified biomimetic method. The characteristics of NGF-CS/HA-coating implants were determined using a scanning electron microscope. After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.

RESULTS

NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA). Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs. Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.

CONCLUSION

Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.

摘要

背景

骨整合是牙种植体咀嚼功能的前提。神经生长因子（NGF）作为一种神经营养因子，可诱导骨愈合。然而，NGF-硫酸软骨素（CS）/羟基磷灰石（HA）-涂层复合植入物对骨整合和神经支配的影响尚不完全清楚。

材料和方法

采用改良仿生法制备 NGF-CS/HA-涂层复合植入物。通过扫描电子显微镜确定 NGF-CS/HA-涂层植入物的特性。将 NGF-CS/HA-涂层植入物置于比格犬下颌骨后，通过 X 射线、Micro-CT、最大拔出力、双重荧光染色、甲苯胺蓝染色、DiI 神经示踪剂、免疫组织化学和 RT-qPCR 检测评估植入物周围组织的早期骨整合和神经支配。

结果

成功制备了 NGF-CS/HA-涂层复合植入物，其呈现出具有主要成分（Ti 和 HA）的多孔网状结构。此外，我们发现植入 NGF-CS/HA-涂层植入物显著改变了下颌骨组织的形态，并提高了 Beagle 犬下颌骨的最大输出、MAR、BIC 和神经纤维。此外，我们证明植入 NGF-CS/HA-涂层植入物还显著上调了 Beagle 犬下颌骨中 NGF、成骨分化和神经生成分化相关基因的水平。

结论

植入 NGF-CS/HA-涂层复合植入物对 Beagle 犬下颌骨植入物周围组织的早期骨整合和神经再生具有显著的诱导作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/7ae65a69216b/13018_2020_2177_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/d552d3aef780/13018_2020_2177_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/5819fc81dcca/13018_2020_2177_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/ffc2b4ae05e4/13018_2020_2177_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/72ea5db685f1/13018_2020_2177_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/7409779dacb6/13018_2020_2177_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/7ae65a69216b/13018_2020_2177_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/d552d3aef780/13018_2020_2177_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/5819fc81dcca/13018_2020_2177_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/ffc2b4ae05e4/13018_2020_2177_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/72ea5db685f1/13018_2020_2177_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/7409779dacb6/13018_2020_2177_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b835/7805124/7ae65a69216b/13018_2020_2177_Fig6_HTML.jpg

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