Schröder J M, Christophers E
J Invest Dermatol. 1986 Jul;87(1):53-8. doi: 10.1111/1523-1747.ep12523566.
Scales from patients with nonpustular psoriasis were investigated for the presence of peptides capable of activating functional activities in human polymorphonuclear leukocytes (PMNL). Two compounds with similar molecular weight (12,500 and 15,000) were isolated which markedly stimulated PMNL functional activities including chemotaxis, generation of superoxide radical anion (O-2), and liberation of beta-glucuronidase as a marker enzyme. As revealed by ion-exchange and subsequent radioimmunoassay followed by chromatofocusing, one peptide proved to be the desarginated form of the complement split product C5ades arg. No C5a was detectable. As a second psoriatic scale chemotaxin we isolated an anionic neutrophil-activating peptide (ANAP) which shows a single isoelectric point at pH 6.8. This peptide shares some of the characteristics of epidermal cell-derived thymocyte-activating factor and interleukin 1 and, as shown by deactivation experiments, it cross-reacts with a monocyte-derived cytokine. The 2 newly described neutrophil-activating peptides (C5ades arg and ANAP) may play an important role in the psoriatic tissue reaction.
对非脓疱型银屑病患者的鳞屑进行了研究，以检测其中是否存在能够激活人多形核白细胞（PMNL）功能活性的肽。分离出了两种分子量相似（12,500和15,000）的化合物，它们能显著刺激PMNL的功能活性，包括趋化性、超氧阴离子自由基（O-2）的产生以及作为标记酶的β-葡萄糖醛酸酶的释放。通过离子交换、随后的放射免疫测定以及层析聚焦分析发现，其中一种肽被证明是补体裂解产物C5ades arg的去精氨酸形式。未检测到C5a。作为第二种银屑病鳞屑趋化因子，我们分离出了一种阴离子中性粒细胞激活肽（ANAP），其在pH 6.8处显示单一的等电点。这种肽具有一些表皮细胞衍生的胸腺细胞激活因子和白细胞介素1的特征，并且如失活实验所示，它与一种单核细胞衍生的细胞因子发生交叉反应。新描述的这两种中性粒细胞激活肽（C5ades arg和ANAP）可能在银屑病组织反应中起重要作用。
