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一种用于多种利什曼原虫生长的组织培养系统：生长动力学和药物敏感性

A tissue culture system for the growth of several species of Leishmania: growth kinetics and drug sensitivities.

作者信息

Martinez S, Looker D L, Marr J J

机构信息

Departamento de Medicina Interna, Universidad del Cauca, Popayan, Colombia, South America.

出版信息

Am J Trop Med Hyg. 1988 Mar;38(2):304-7. doi: 10.4269/ajtmh.1988.38.304.

DOI:10.4269/ajtmh.1988.38.304
PMID:2833127
Abstract

We have developed a simple in vitro method of infecting a continuous human macrophage cell line (U937) with promastigotes of several species of Leishmania. These include L. braziliensis braziliensis, L. b. panamensis, L. donovani, L. mexicana mexicana, L. m. pifanoi, L. tropica, and L. major. The growth kinetics of these species are presented as well as drug sensitivity data. The U937 cell system can be used to determine drug efficacy and eliminates the need to use amastigotes from animal tissues to infect the tissue culture.

摘要

我们已经开发出一种简单的体外方法，用几种利什曼原虫的前鞭毛体感染连续传代的人巨噬细胞系（U937）。这些利什曼原虫包括巴西利什曼原虫巴西亚种、巴拿马利什曼原虫、杜氏利什曼原虫、墨西哥利什曼原虫墨西哥亚种、皮氏利什曼原虫、热带利什曼原虫和硕大利什曼原虫。文中呈现了这些虫种的生长动力学以及药物敏感性数据。U937细胞系统可用于确定药物疗效，并且无需使用来自动物组织的无鞭毛体来感染组织培养物。

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A tissue culture system for the growth of several species of Leishmania: growth kinetics and drug sensitivities.一种用于多种利什曼原虫生长的组织培养系统：生长动力学和药物敏感性
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引用本文的文献

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Axenically grown amastigotes of Leishmania infantum used as an in vitro model to investigate the pentavalent antimony mode of action.将婴儿利什曼原虫的无菌培养无鞭毛体用作体外模型，以研究五价锑的作用模式。
Antimicrob Agents Chemother. 1998 Dec;42(12):3097-102. doi: 10.1128/AAC.42.12.3097.
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Dichotomy of the human T cell response to Leishmania antigens. II. Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients.
人类T细胞对利什曼原虫抗原反应的二分法。II. 治愈的内脏利什曼病患者细胞对gp63无反应或呈Th2样反应，而对脂磷壁酸相关蛋白呈Th1样反应
Clin Exp Immunol. 1994 Jun;96(3):416-21. doi: 10.1111/j.1365-2249.1994.tb06044.x.
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Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections.皮肤利什曼病患者对利什曼原虫抗原的T细胞反应二分法；Th1活性的缺乏或不足与严重感染相关。
Clin Exp Immunol. 1995 May;100(2):239-45. doi: 10.1111/j.1365-2249.1995.tb03660.x.
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