Wiecek Magdalena, Szymura Jadwiga, Maciejczyk Marcin, Kantorowicz Malgorzata, Szygula Zbigniew
Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, University of Physical Education in Krakow, Krakow, Poland.
Department of Clinical Rehabilitation, Faculty of Motor Rehabilitation, University of Physical Education in Krakow, Krakow, Poland.
Front Physiol. 2018 Jul 27;9:1006. doi: 10.3389/fphys.2018.01006. eCollection 2018.
Physical exercise changes redox balance in the blood. The study aim is to determine gender-related differences in enzymatic antioxidant defense [superoxide dismutase, catalase (CAT), and glutathione peroxidase (GPx)] during the initial period following anaerobic exercise and 24 h after its completion. Young, non-training participants (10 women and 10 men) performed a single anaerobic exercise, which was a 20-s maximal cycling sprint test. Blood was collected before and after completing the anaerobic exercise, i.e., after 3, 15, 30, and 60 min and after 24 h. Lactate concentration, and the superoxide dismutase, CAT, and GPx activity were determined. The results were adapted to the changes in plasma volume. Anaerobic exercise induced a significant increase in lactate concentration, similar among both sexes. Anaerobic exercise evokes identical changes in the activity of antioxidant enzymes in the blood plasma of women and men, which is dependent on anaerobic capacity. In the early phase of restitution, the activity of antioxidant enzymes decreases; 24 h after anaerobic exercise, GPx activity in the blood plasma of women and men is higher than before the exercise. There are no gender-related differences concerning changes in plasma antioxidant activity after anaerobic exercise. Depending on the antioxidant enzyme, changes of activity differ in time after the end of the anaerobic exercise.
体育锻炼会改变血液中的氧化还原平衡。本研究旨在确定无氧运动初始阶段及结束后24小时内，酶促抗氧化防御系统[超氧化物歧化酶、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）]中与性别相关的差异。年轻的非训练参与者（10名女性和10名男性）进行了一次无氧运动，即20秒的最大强度自行车冲刺测试。在完成无氧运动之前和之后，即3、15、30和60分钟后以及24小时后采集血液。测定乳酸浓度以及超氧化物歧化酶、CAT和GPx的活性。结果根据血浆容量的变化进行了调整。无氧运动导致乳酸浓度显著升高，男女之间相似。无氧运动引起男女血浆中抗氧化酶活性的相同变化，这取决于无氧能力。在恢复的早期阶段，抗氧化酶的活性降低；无氧运动24小时后，男女血浆中的GPx活性高于运动前。无氧运动后血浆抗氧化活性的变化不存在与性别相关的差异。根据抗氧化酶的不同，无氧运动结束后活性的变化在时间上有所不同。
