Kaminsky L S, McHugh T, Stites D, Volberding P, Henle G, Henle W, Levy J A
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5535-9. doi: 10.1073/pnas.82.16.5535.
A rapid, sensitive indirect immunofluorescence assay has been developed for detection of antibodies to the acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV). The human T-cell line HUT-78 was chronically infected with ARV-2 and used to detect antibodies to virus-specific cytoplasmic antigens. Because the helper T-cell marker Leu-3 is substantially reduced in this cell line after ARV infection, it appears to be an important receptor for virus infection. Nearly all patients with AIDS and most cases with related conditions showed antibodies against ARV. Some healthy individuals in risk groups for developing AIDS also had antibodies to the agent. In contrast, no antibodies to the virus were found in any individuals outside the risk groups for developing AIDS or with diseases other than those associated with AIDS. The titers of antibodies to ARV and to Epstein-Barr virus varied independently from each other. The level of anti-ARV antibodies in a patient's serum was found to reflect the severity of the disease; it was lower in individuals with more severe manifestations. Taken together, these data support the role of ARV in AIDS and its related disorders.
已开发出一种快速、灵敏的间接免疫荧光测定法，用于检测针对获得性免疫缺陷综合征（AIDS）相关逆转录病毒（ARV）的抗体。人T细胞系HUT-78被ARV-2慢性感染，并用于检测针对病毒特异性细胞质抗原的抗体。由于在ARV感染后该细胞系中的辅助性T细胞标志物Leu-3大幅减少，它似乎是病毒感染的重要受体。几乎所有艾滋病患者以及大多数相关病症患者均显示出针对ARV的抗体。一些处于患艾滋病风险组的健康个体也有针对该病原体的抗体。相比之下，在患艾滋病风险组之外的任何个体或患有除与艾滋病相关疾病以外其他疾病的个体中均未发现针对该病毒的抗体。针对ARV和爱泼斯坦-巴尔病毒的抗体滴度彼此独立变化。发现患者血清中抗ARV抗体水平反映疾病的严重程度；在表现更严重的个体中该水平较低。综上所述，这些数据支持ARV在艾滋病及其相关病症中的作用。
