Singh Hina, Du Juan, Singh Priyanka, Yi Tae Hoo
Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
College of Food and Bioengineering, Zhengzhou University of Light Industry, Henan Province Collaborative Innovation Center for Food Production and Safety, Zhengzhou 450001, China.
J Pharm Anal. 2018 Aug;8(4):258-264. doi: 10.1016/j.jpha.2018.04.004. Epub 2018 Apr 20.
Silver nanoparticles (AgNPs) are known to have bacteriostatic and bactericidal effects. The present study highlights the extracellular synthesis of AgNPs and its antibacterial properties. The AgNPs were synthesized using sp. THG-LS1.4 strain which had been isolated from soil. The AgNPs were characterized by field emission-transmission electron microscopy (FE-TEM), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, and particle size distribution (DLS). The AgNPs displayed maximum absorbance at 412 nm and were irregular in shape ranging from 10 to 40 nm. The XRD spectroscopy results demonstrated the crystalline nature of nanoparticles. The AgNPs showed antimicrobial activity against , , , , and . Furthermore, the AgNPs were also evaluated for their increased antibacterial activities with various antibiotics against , and . Additionally, AgNPs showd biofilm inhibition activity. The biosynthesized AgNPs were found to be a potent agent against tested pathogens. More importantly, we highlight the applications of AgNPs as an antimicrobial agent.
已知银纳米颗粒（AgNPs）具有抑菌和杀菌作用。本研究重点介绍了AgNPs的胞外合成及其抗菌特性。使用从土壤中分离出的sp. THG-LS1.4菌株合成了AgNPs。通过场发射透射电子显微镜（FE-TEM）、X射线衍射（XRD）、傅里叶变换红外（FT-IR）光谱和粒度分布（DLS）对AgNPs进行了表征。AgNPs在412nm处显示出最大吸光度，形状不规则，大小在10至40nm之间。XRD光谱结果证明了纳米颗粒的晶体性质。AgNPs对 、 、 、 、 和 显示出抗菌活性。此外，还评估了AgNPs与各种抗生素联合对 、 和 的增强抗菌活性。此外，AgNPs还表现出生物膜抑制活性。发现生物合成的AgNPs是对抗测试病原体的有效剂。更重要的是，我们强调了AgNPs作为抗菌剂的应用。
