a Department of Oriental Medicine Biotechnology , Ginseng Bank, College of Life Science, Kyung Hee University , Seocheon-dong, Giheung-gu , Yongin-si , Gyeonggi-do , Republic of Korea.
b Graduate School of Biotechnology , College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
Artif Cells Nanomed Biotechnol. 2016 Dec;44(8):1949-1957. doi: 10.3109/21691401.2015.1115410. Epub 2015 Dec 24.
The study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of Panax ginseng, an herbal medicinal plant. The reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°C, respectively. The developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. The nanoparticles were further characterized by Ultraviolet-visible spectroscopy (UV-vis) which showed the relevant peak for gold and silver nanoparticles at 578 and 420 nm, correspondingly. Field-emission transmission electron microscopy (FE-TEM) displayed the spherical shape of monodispersed nanoparticles. FE-TEM revealed that the gold nanoparticles were 10-20 nm and silver nanoparticles were 5-15 nm. The energy dispersive X-ray (EDX) and elemental mapping results indicated the maximum distribution of gold and silver elements in the respective nanoproducts, which further corresponds the purity. Further, the X-ray diffraction (XRD) results confirm the crystalline nature of synthesized nanoparticles. The biosynthesized AgNPs served as an efficient antimicrobial agent at 3 μg concentration against many pathogenic strains for instance, Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus anthracis and Bacillus cereus. In addition, AgNPs showed complete inhibition of biofilm formation by S. aureus and Pseudomonas aeruginosa at 4 μg/ml concentration. Moreover, the AuNPs and AgNPs found as a potent anticoagulant agent. Thus, the study claims the rapid synthesis of gold and silver nanoparticles by fresh P. ginseng leaf extract and its biological applications.
该研究强调了通过草本药用植物人参的新鲜叶片合成金纳米粒子和银纳米粒子。在 80°C 下，金纳米粒子在 3 分钟内，银纳米粒子在 45 分钟内形成，分别由氯化金和硝酸银还原而成。所开发的方法快速、简便、环保，最重要的是非常经济实惠，不需要后续进行纳米粒子的还原或稳定化处理。纳米粒子进一步通过紫外可见光谱（UV-vis）进行了表征，分别在 578nm 和 420nm 处显示出金和银纳米粒子的相关峰。场发射透射电子显微镜（FE-TEM）显示出单分散纳米粒子的球形形状。FE-TEM 显示金纳米粒子的粒径为 10-20nm，银纳米粒子的粒径为 5-15nm。能量色散 X 射线（EDX）和元素映射结果表明，金和银元素在各自的纳米产物中分布最多，这进一步对应了纯度。此外，X 射线衍射（XRD）结果证实了合成纳米粒子的结晶性质。生物合成的 AgNPs 在 3μg 浓度下对多种病原菌（如大肠杆菌、肠炎沙门氏菌、副溶血性弧菌、金黄色葡萄球菌、炭疽芽孢杆菌和蜡状芽孢杆菌）具有高效的抗菌作用。此外，AgNPs 在 4μg/ml 浓度下完全抑制了金黄色葡萄球菌和铜绿假单胞菌的生物膜形成。此外，AuNPs 和 AgNPs 被发现是一种有效的抗凝剂。因此，该研究声称通过新鲜人参叶片提取物快速合成金和银纳米粒子及其生物应用。
