Huq Md Amdadul, Akter Shahina
Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong 17546, Korea.
Department of Food Science and Biotechnology, Gachon University, Seongnam 461-701, Korea.
Materials (Basel). 2021 May 18;14(10):2615. doi: 10.3390/ma14102615.
In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive and Gram-negative bacteria . Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) analysis. The UV-Vis spectral analysis showed the absorption maxima at 466 nm which assured the synthesis of AgNPs. The FE-TEM analysis revealed the spherical shape of nanoparticles with the size range from 13 to 27 nm. The EDX and XRD analysis ensured the crystalline nature of biosynthesized AgNPs. The FTIR analysis revealed the involvement of different biomolecules for the synthesis of AgNPs as reducing and capping agents. The bacterial-mediated synthesized AgNPs inhibited the growth of pathogenic strains and and developed a clear zone of inhibition (ZOI). The MIC and MBC for both pathogens were 12.5 µg/mL and 25 µg/mL, respectively. Moreover, field emission scanning electron microscopy analysis revealed that the synthesized AgNPs can destroy the outer membrane and alter the cell morphology of treated pathogens, leading to the death of cells. This study concludes the eco-friendly, facile and rapid synthesis of AgNPs using MAHUQ-43 and synthesized AgNPs showed excellent antimicrobial activity against both Gram-positive and Gram-negative pathogens.
在本研究中，对利用细菌菌株MAHUQ - 43的培养上清液生物合成的银纳米颗粒（AgNPs）进行了表征，并研究了其对革兰氏阳性菌和革兰氏阴性菌的抗菌活性。通过紫外可见（UV - Vis）分光光度计、场发射透射电子显微镜（FE - TEM）、能量色散X射线（EDX）、X射线衍射（XRD）、傅里叶变换红外（FTIR）光谱以及动态光散射（DLS）分析对细菌介导合成的AgNPs进行了表征。UV - Vis光谱分析显示在466 nm处有吸收最大值，这确保了AgNPs的合成。FE - TEM分析揭示了纳米颗粒呈球形，尺寸范围为13至27 nm。EDX和XRD分析确定了生物合成的AgNPs的晶体性质。FTIR分析表明不同生物分子作为还原剂和封端剂参与了AgNPs的合成。细菌介导合成的AgNPs抑制了致病菌株的生长，并形成了清晰的抑菌圈（ZOI）。两种病原体的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为12.5 µg/mL和25 µg/mL。此外，场发射扫描电子显微镜分析表明，合成的AgNPs可破坏处理后病原体的外膜并改变其细胞形态，导致细胞死亡。本研究得出结论，利用MAHUQ - 43可实现银纳米颗粒的环保、简便且快速合成，并且合成的银纳米颗粒对革兰氏阳性和革兰氏阴性病原体均表现出优异的抗菌活性。
