From the Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran.
ASAIO J. 2018 Sep/Oct;64(5):e115-e122. doi: 10.1097/MAT.0000000000000781.
Over the last few decades, great advancements have been achieved in the field of bone tissue engineering (BTE). Containing a great number of growth factors needed in the process of osteogenesis, platelet rich plasma (PRP) has gained a great deal of attention. However, due to the contradictory results achieved in different studies, its effectiveness remains a mystery. Therefore, in this study, we investigated in vitro performance of co-electrospun PRP/poly ether sulfone/poly(vinyl) alcohol (PRP/PES/PVA) composite scaffolds for the osteogenic differentiation of human adipose-derived mesenchymal stem cells. The activated PRP was mixed with PVA solution to be used alongside PES solution for the electrospinning process. Fourier transform infrared spectroscopy, scanning electron microscopy and tensile tests were performed to evaluate the scaffolds. After confirmation of sustained release of protein, osteogenic potential of the co-electrospun PRP/polymer scaffolds was evaluated by measuring relative gene expression, calcium content, and alkaline phosphatase (ALP) activity. Alizarin red and Hematoxylin and Eosin staining were performed as well. The results of ALP activity and calcium content demonstrated the effectiveness of PRP when combined with PRP-incorporated scaffold in comparison with the other tested groups. In addition, the results of tensile mechanical testing indicated that addition of PRP improves the mechanical properties. Taking these results into account, it appears PES/PVA/PRP scaffold treated with PRP 5% enhances osteogenic differentiation most. In conclusion, incorporation of PRP into electrospun PES/PVA scaffold in this study had a positive influence on osteogenic differentiation of AdMSCs, and thus it may have great potential for BTE applications.
在过去的几十年中，骨组织工程（BTE）领域取得了巨大的进展。富含成骨过程中所需的大量生长因子的富血小板血浆（PRP）引起了广泛关注。然而，由于不同研究中得出的结果相互矛盾，其有效性仍然是个谜。因此，在这项研究中，我们研究了共电纺 PRP/聚醚砜/聚乙烯醇（PRP/PES/PVA）复合支架在人脂肪间充质干细胞成骨分化中的体外性能。将激活的 PRP 与 PVA 溶液混合，与 PES 溶液一起用于电纺过程。通过傅里叶变换红外光谱、扫描电子显微镜和拉伸试验对支架进行了评估。在确认蛋白质持续释放后，通过测量相对基因表达、钙含量和碱性磷酸酶（ALP）活性来评估共电纺 PRP/聚合物支架的成骨潜力。还进行了茜素红和苏木精-伊红染色。ALP 活性和钙含量的结果表明，与其他测试组相比，PRP 与 PRP 掺入支架结合使用时，PRP 更有效。此外，拉伸力学测试的结果表明，添加 PRP 可改善机械性能。考虑到这些结果，似乎用 5%PRP 处理的 PES/PVA/PRP 支架增强了成骨分化。总之，本研究中 PRP 掺入电纺 PES/PVA 支架对 AdMSCs 的成骨分化有积极影响，因此它在 BTE 应用中可能具有很大的潜力。
